Ib Kauj Ruam Ceev RT-qPCR Probe Premix-UNG
Cat No: HCR5143A
Ib Kauj Ruam RT-qPCR Probe Kit (FOR FAST) yog ib qho kev sojntsuam-raws li RT-qPCR ceev nrhiav cov khoom siv rau ib leeg-plex lossis multiplex quantitative PCR siv RNA ua tus qauv (xws li RNA virus).Cov khoom no siv lub cim tshiab ntawm cov tshuaj tiv thaiv-hloov kho Taq DNA Polymerase thiab ib-kauj ruam mob siab rau Reverse Transcriptase, nrog rau kev ua kom tsis zoo rau kev ua kom nrawm nrawm, uas muaj qhov nrawm nrawm dua, kev ua kom zoo dua qub thiab qhov tshwj xeeb.Nws txhawb nqa kev sib npaug ntawm ob qho tib si plex thiab multiplex ntawm cov qauv qis thiab siab concentration hauv lub sijhawm luv luv.
Cheebtsam
1. 5 × RT-qPCR tsis muaj (U+)
2. Enzyme mix (U+)
Nco tseg:
a.5 × RT-qPCR buffer (U+) suav nrog dNTP thiab Mg2+;
b.Enzyme mix (U+) suav nrog rov qab transcriptase, Kub Pib Taq DNA polymerase, RNase inhibitor thiab UDG;
c.Siv RNase-Dawb cov lus qhia, EP raj, thiab lwm yam.
Ua ntej siv, sib tov kom huv si 5 × RT-qPCR tsis (U+).Yog tias muaj nag lossis daus tom qab thawing, tos kom tsis txhob rov qab mus rau chav sov, sib tov thiab yaj, thiab siv lawv li qub.
Kev cia khoom
Cov khoom xa tuaj nrog cov dej khov qhuav thiab tuaj yeem khaws cia ntawm -25 ~ -15 ℃ rau 1 xyoos.
Cov lus qhia
1. Kev tshwm sim Qhov system
| Cheebtsam | Ntim (20 μL tshuaj tiv thaiv) |
| 2 × RT-qPCR tsis | 4l miv |
| Enzyme mix (U+) | 0,8l wb |
| Primer Forward | 0.1 ~ 1.0μM |
| Primer Rov qab | 0.1 ~ 1.0μM |
| TqMan Probe | 0.05 ~ 0.25μM |
| Template | X μL |
| RNase-dawb Dej | mus txog 25μL |
Lus Cim: Cov tshuaj tiv thaiv ntim yog 10-50μL.
2. Cycling Protocol (Standard)
| Lub voj voog kauj ruam | Temp. | Sijhawm | Lub voj voog |
| Rov qab Transcription | 55 ℃ | 10 feeb | 1 |
| Initial Denaturation | 95 ℃ | 30 sec | 1 |
| Denaturation | 95 ℃ | 10 sec | 45 |
| Annealing / Extension | 60 ℃ | 30 sec |
| Cycling Protocol (Sai sai) Lub voj voog kauj ruam |
Temp. |
Sijhawm |
Lub voj voog |
| Rov qab Transcription | 55 ℃ | 5 feeb | 1 |
| Initial Denaturation | 95 ℃ | 5 s ib | 1 |
| Denaturation | 95 ℃ | 3 s ib | 43 |
| Annealing / Extension | 60 ℃ | 10 s ib |
Nco tseg:
a.Qhov ntsuas kub rov qab yog nyob nruab nrab ntawm 50 ℃ txog 60 ℃, nce qhov kub thiab txias pab kom nthuav dav cov qauv thiab cov qauv CG siab;
b.Qhov zoo tshaj plaws annealing kub yuav tsum tau hloov kho raws li tus nqi Tm ntawm primer, thiab xaiv lub sij hawm luv tshaj plaws rau fluorescence teeb liab sau raws li Real Time PCR ntsuas.
Sau ntawv
Thov hnav PPE tsim nyog, xws li lub tsho tiv no thiab hnab looj tes, txhawm rau ua kom koj noj qab haus huv thiab nyab xeeb!
