Phosphatase Alkaline (ALP)
Kev piav qhia
Alkaline Phosphatase yog muab los ntawm ib tug recombinant E. coli hom uas nqa cov noob TAB5.Lub enzyme catalyzes dephosphorylation ntawm 5' thiab 3' kawg ntawm DNA thiab RNA phosphomonoesters.Tsis tas li ntawd, nws hydrolyses ribose, nrog rau deoxyribonucleoside triphosphates (NTPs thiab dNTPs).TAB5 Alkaline Phosphatase ua rau 5' protruding, 5' recessed thiab blunt kawg.Phosphatase tuaj yeem siv tau rau hauv ntau daim ntawv thov kev lom neeg molecular, xws li cloning lossis sojntsuam kawg daim ntawv lo kom tshem tawm cov phosphorylated kawg ntawm DNA lossis RNA.Hauv kev sim cloning, dephosphorylation tiv thaiv cov linearized plasmid DNA los ntawm tus kheej-ligation.Nws tseem tuaj yeem degrade unincorporated dNTPs hauv PCR cov tshuaj tiv thaiv los npaj cov qauv rau DNA sequencing.Lub enzyme yog tag nrho thiab irreversibly inactivated los ntawm cua sov ntawm 70 ° C rau 5 feeb, yog li ua kom tshem tawm cov phosphatase ua ntej ligation los yog xaus daim ntawv lo tsis tsim nyog.
Kev siv
1.Alkaline phosphatase ua ke rau cov proteins (antibodies, streptavidin thiab lwm yam,) tuaj yeem txheeb xyuas cov hom phiaj molecules, thiab tuaj yeem siv rau hauv ELISA, WB thiab kuaj histochemical;
2.Alkaline phosphatase tuaj yeem siv los dephosphorize 5 '-terminal ntawm DNA lossis RNA los tiv thaiv kev sib txuas ntawm tus kheej;
3.Qhov saum toj no dephosphorylated DNA lossis RNA tuaj yeem sau los ntawm xov tooj cua-labeled phosphates (ntawm T4 poly-nucleotide kinase)
Chemical Structure
Specification
| Cov khoom kuaj | Specifications |
| Kev ua enzyme | 5 U / L |
| Endonuclease Activity | Tsis pom |
| Kev Ua Haujlwm Exonuuclease | Tsis pom |
| Kev Ua Haujlwm Nicking | Tsis pom |
| RNase Kev Ua Haujlwm | Tsis pom |
| E.coli DNA | ≤1 daim / 5U |
| Endotoxin | LAL-Test, ≤10EU/mg |
| Purity | ≥95% |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:AmbIent
Cia:Khaws ntawm 2-8 ° C
Pom zoo rov sim duaLub neej:2 xyoo
