RT-LAMP Fluorescent (Lyophilized pob)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) muaj cov tshuaj tiv thaiv tsis zoo, Bst DNA polymerase thiab kub-tiv thaiv rov qab transcriptase, lyophilized tiv thaiv, thiab fluorescent zas xim.Cov khoom yog nyob rau hauv daim ntawv ntawm lyophilized hlaws dai, thiab tsuas yog primers thiab templates yuav tsum tau ntxiv rau siv.
Reagent Cheebtsam
RT-LAMP Fluorescent Master Mix (Lyophilized Hmoov)
Kev siv
Rau DNA lossis RNA isothermal amplification
Kev cia khoom
Kev thauj mus los thiab khaws cia ntawm 2 ~ 8 ° C.
Lub neej txee: 12 lub hlis.
raws tu qauv
1. Tshem tawm cov xov tooj ntawm cov lyophilized hlaws raws li tus naj npawb ntawm kev xeem.
2. Kev npaj tshuaj tiv thaiv
| Cheebtsam | Noj |
| RT-LAMP Fluorescent Master Mix (Lyophilized hmoov) | 2 zoj |
| Fluorescent xim | x m li |
| 10 × Primer Mix a | 5l miv |
| Templates DNA/RNA b | n miv l |
| Tag nrho | 50l ua |
Nco tseg:
1) a.10 × Primer Mix: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) ib.Cov qauv nucleic acid tau pom zoo kom yaj hauv DEPC dej.
Hyasen Biotechnology Co., Ltd
3) Cov kev pab cuam cuam tshuam
| Kub | Sijhawm | Lub voj voog |
| 65 ℃ | 60s ib a | 30 |
Nco tseg:
1. Sau cov cim fluorescent
Sau ntawv
1. Cov tshuaj tiv thaiv kub tuaj yeem ua kom zoo ntawm 62 ° C thiab 68 ° C raws li cov primertej yam kev mob;
2. Qhov kev sim yuav tsum ua kom zoo rau kev ua haujlwm, suav nrog kev npaj cov tshuaj tiv thaiv kab mob,qauv ua thiab dosing;
3. Txhawm rau kom tsis txhob muaj kev sib kis, nws raug nquahu kom npaj cov tshuaj tiv thaiv kab mob hauv lub rooj ua kom huv huvthiab ntxiv cov qauv rau lub fume hood hauv lwm chav kom tsis txhob muaj kev cuam tshuam tsis zoo.
