2 × PCR Master Mix (tsis muaj Dye)
PCR Master Mix yog ib hom qauv PCR premixed tov uas yog npaj siv, suav nrog Taq DNA Polymerase, dNTP mix MgCl2 thiab optimized buffer.Thaum lub sij hawm cov tshuaj tiv thaiv, tsuas yog cov primer thiab template yuav muab ntxiv rau amplification, uas zoo heev simplifies lub lag luam cov kauj ruam ntawm kev sim.Cov khoom no muaj cov stabilizers zoo heev thiab tuaj yeem khaws cia rau 3 lub hlis ntawm 4 ℃.Cov khoom PCR muaj 3'-dA protrusion thiab tuaj yeem yooj yim cloned rau hauv T vector.
Kev cia khoom
Cov khoom yuav tsum khaws cia ntawm -25 ℃ ~ -15 ℃ rau ob xyoos.
Specifications
| Kev ncaj ncees (vs.Taq) | 1 × |
| Pib kub | No |
| Overhang | 3'-A |
| Polymerase | Cov DNA Polymerase |
| Hom tshuaj tiv thaiv | SuperMix lossis Master Mix |
| Teb ceev | Txuj |
| Yam khoom | PCR Master Mix (2 ×) |
Cov lus qhia
1.Reaction System
| Cheebtsam | Loj (μL) |
| Template DNA | Tsim nyog |
| Primer 1 (10 μmol / L) | 2 |
| Primer 2 (10 μmol / L) | 2 |
| PCR Master Mix | 25 |
| ddH ua2O | Rau 50 |
2.Amplification raws tu qauv
| Lub voj voog kauj ruam | Qhov kub thiab txias (°C) | Sijhawm | Lub voj voog |
| Pre-denaturation | 94 ℃ | 5 feeb | 1 |
| Denaturation | 94 ℃ | 30 sec | 35 |
| Annealing | 50-60 ℃ | 30 sec | |
| Txuas ntxiv | 72 ℃ | 30-60 sec/kb | |
| Kawg Extension | 72 ℃ | 10 feeb | 1 |
Nco tseg:
1) Cov qauv siv: 50-200 ng genomic DNA;0.1-10 ng plasmid DNA.
2) Mg2+concentration: Cov khoom no muaj 3 mM ntawm MgCl2 haum rau feem ntau cov tshuaj PCR.
3) Annealing kub: Thov xa mus rau qhov theoretical Tm tus nqi ntawm Primers.Lub annealing kub tuaj yeem teem rau 2-5 ℃ qis dua tus nqi theoretical ntawm primer.
4) Lub Sijhawm Ntxiv: Rau kev txheeb xyuas molecular, 30 sec / kb raug pom zoo.Rau gene cloning, 60sec/kb raug pom zoo.
Sau ntawv
1.Rau koj txoj kev nyab xeeb thiab kev noj qab haus huv, thov hnav lub tsho (lab) thiab cov hnab looj tes pov tseg rau kev ua haujlwm.
2.Rau kev tshawb fawb siv xwb!
