Recombinant Deoxyribonuclease I (DNase I, RNase-dawb)
Kev piav qhia
Recombinant Deoxyribonuclease I, yog ib qho endonuclease uas tuaj yeem zom ib leeg lossis ob-stranded DNA.Nws hydrolyzes phosphodiester bonds los tsim cov mono- thiab oligodeoxynucleotides uas muaj 5'-phosphate pawg thiab 3'-OH pawg.Qhov zoo tshaj plaws pH ua haujlwm ntawm DNase I yog 7-8.Qhov kev ua ntawm DNase kuv nyob ntawm Ca2+ thiab tuaj yeem qhib tau los ntawm divalent hlau ions xws li Co2+, Mn2+, Zn2+, thiab lwm yam. Nyob rau hauv lub xub ntiag ntawm Mg2+, DNase kuv tau randomly txiav ob-stranded DNA ntawm txhua qhov chaw;thaum nyob rau hauv lub xub ntiag ntawm Mn2+, DNase kuv tuaj yeem txiav DNA ob zaug tso rau ntawm tib qhov chaw, tsim blunt kawg lossis 1-2 nuclei Nplaum xaus nrog nucleotide overhangs.Cov enzyme no tau muab los ntawm cov kab mob E. coli recombinant, tsis muaj RNase, thiab tuaj yeem siv rau kev ua ntawm ntau cov qauv RNA.
Cov qauv tshuaj
Daim ntawv thov
RNA rho tawm: npaj RNA yam tsis muaj DNA;
Tom qab hauv vitro transcription nrog RNA polymerase, xws li T7 RNA Polymerase (Cat # 10618), nws yog siv los tshem tawm cov qauv DNA;
Npaj DNA-dawb RNA ua ntej RT-PCR thiab RT-qPCR;
Siv ua ke nrog DNA Polymerase I (Cat # 12903) rau kev sau npe DNA los ntawm kev txhais lus nick;
Siv hauv kev txheeb xyuas hneev taw los txheeb xyuas DNA-protein kev sib cuam tshuam;
Tsim ib lub tsev qiv ntawv random fragment;
Nyob rau hauv TUNEL nrhiav pom ntawm apoptosis, ib feem sheared genomic DNA tau siv los tswj qhov zoo.
Shipping thiab Cia
Kev thauj mus los:Cov hnab ntim dej khov
Kev cia khoom:Khaws hauv -20 ℃
Lub neej txee:2 xyoo
Nco tseg:
1.Enzymes yuav tsum muab khaws cia rau hauv lub thawv dej khov lossis hauv lub da dej dej khov thaum siv, thiab yuav tsum khaws cia ntawm -20 ° C tam sim tom qab siv.
2.Rau koj txoj kev nyab xeeb thiab kev noj qab haus huv, thov hnav cov khoom tiv thaiv tus kheej (PPE), xws li cov tsho hauv chav kuaj thiab cov hnab looj tes pov tseg, thaum ua haujlwm nrog cov khoom no.
