DNase I (Rnase Free) (5u/ul)
Cat No: HC4007A
DNase I (Deoxyribonuclease I) yog ib qho endodeoxyribonuclease uas tuaj yeem zom cov DNA ib leeg lossis ob npaug.Nws paub thiab cleaves phosphodiester bonds los tsim monodeoxynucleotides los yog ib leeg-los yog ob-stranded oligodeoxynucleotides nrog phosphate pawg ntawm 5'-terminal thiab hydroxyl ntawm 3'-terminal.Qhov kev ua ntawm DNase kuv nyob ntawm Ca2+thiab tuaj yeem qhib tau los ntawm divalent hlau ions xws li Mn2+thiab Zn2+.5m cav2+tiv thaiv cov enzyme los ntawm hydrolysis.Hauv lub xub ntiag ntawm Mg2+, lub enzyme yuav random lees paub thiab cleave txhua qhov chaw ntawm txhua strand ntawm DNA.Hauv lub xub ntiag ntawm Mn2+, ob kab ntawm DNA tuaj yeem raug lees paub ib txhij thiab cleaved ntawm yuav luag tib lub xaib kom tsim cov kab kawg DNA tawg lossis nplaum kawg DNA tawg nrog 1-2 nucleotides protruding.
Cheebtsam
| Lub npe | 0.1 TSI | 1 KU | 5 KU | 50 KUB |
| DNase I, RNase-dawb | 20 l m | 200 mL | 1 mL ib | 10 ml ib |
| 10 × DNase Kuv Buffer | 1 mL ib | 1 mL ib | 5 × 1ml | 5 × 10 ml |
Cia tej yam kev mob
-25 ℃ ~ -15 ℃ rau cia;Kev thauj mus los hauv qab ntim dej khov.
Cov lus qhia
1. Npaj cov tshuaj tiv thaiv nyob rau hauv RNase-free raj raws li cov proportions teev hauv qab no:
| Cheebtsam | Ntim |
| RNA | X µg |
| 10 × DNase kuv Buffer | 1 m l |
| DNase I, RNase-dawb (5U / μL) | 1 U per µg RNA ① |
| ddH ua2O | Txog li 10μL |
Nco tseg: ①Xam qhov ntim ntawm DNase I uas yuav tsum tau ntxiv raws li tus nqi ntawm RNA.
2. 37 ℃ rau 15 feeb;
3. Ntxiv 0.5M EDTA rau qhov kawg concentration ntawm 2.5mM ~ 5mM, thiab cua sov ntawm 65 ℃ rau 10 feeb kom tsis txhob muaj cov tshuaj tiv thaiv.Cov qauv tuaj yeem siv ncaj qha rau cov tshuaj tiv thaiv tom ntej xws li thim rov qabkev sim.
Unit txhais
Ib chav tsev yog txhais raws li tus nqi ntawm cov enzyme uas yuav degrade tag nrho 1µg ntawm pBR322DNA hauv 10 feeb ntawm 37 ℃.
Kev Tswj Xyuas Zoo
RNase:5U ntawm DNase I nrog 1.6µg MS2 RNA rau 4 teev ntawm 37 ℃ yields tsis degradation liKev txiav txim siab los ntawm agarose gel electrophoresis.
Sau ntawv
1. Thov npaj 0.5MEDTA ntawm koj tus kheej.
2. Siv 1U DNase I ib µg ntawm RNA.Txawm li cas los xij, yog tias RNA tsawg dua 1µg, thov siv 1U DNase I.
3. Thov tso cov enzyme rau dej khov thaum ua haujlwm.
