Ribonuclease A (RNase A)

Ribonuclease A (RNase A) yog ib leeg-stranded polypeptide uas muaj 4 disulfide bonds nrog ib tug molecular hnyav li ntawm 13.7 kDa.RNase A yog ib qho endoribonuclease uas tshwj xeeb degrades ib leeg-stranded RNA ntawm C thiab U residues.Tshwj xeeb, qhov cleavage paub txog phosphodiester daim ntawv cog lus tsim los ntawm 5'-ribose ntawm nucleotide thiab phosphate pawg ntawm 3'-ribose ntawm cov pyrimidine nucleotide uas nyob ib sab, yog li ntawd 2', 3' - Cyclic phosphates yog hydrolyzed rau corresponding. 3'-nucleoside phosphates (piv txwv li, pG-pG-pC-pA-pG yog cleaved los ntawm RNase A los tsim pG-pG-pCp thiab A-PG).RNase A yog qhov ua haujlwm tshaj plaws hauv kev sib tsoo ib leeg-stranded RNA thiab ua haujlwm hauv ntau hom tshuaj tiv thaiv: ntawm cov ntsev tsawg (0 txog 100 mM NaCl), nws tuaj yeem siv los txiav ib leeg-stranded RNA, ob-stranded RNA, thiab RNA strands hauv RNA-DNA hybrids.thaum muaj ntsev ntau (≥0.3 M), RNase A tuaj yeem tshwj xeeb cleave ib leeg-stranded RNA.