Ib Kauj Ruam RT-qPCR cov khoom siv sojntsuam
Kev piav qhia
Ib Kauj Ruam QRT-PCR Probe Cov Khoom Siv yog tsim tshwj xeeb rau qPCR uas ncaj qha siv RNA (xws li tus kab mob RNA) ua qauv.Siv cov gene tshwj xeeb primers (GSP), qhov kev hloov pauv rov qab thiab qPCR tuaj yeem ua tiav hauv ib lub raj, txo cov txheej txheem pipetting thiab kev pheej hmoo ntawm kev sib kis.Nws tuaj yeem ua haujlwm tsis tau ntawm 55 ℃ yam tsis muaj kev cuam tshuam rau kev ua haujlwm thiab rhiab heev ntawm qRT-PCR.Kev sib xyaw ua haujlwm zoo tshaj plaws ntawm HiScript III Reverse Transcriptase thiab kub pib Champagne Taq DNA Polymerase, nrog rau qhov kev ua kom zoo dua qub, qhov kev tshawb pom qhov rhiab heev ntawm Ib Kauj Ruam QRT-PCR Cov Khoom Siv Tshawb Fawb tuaj yeem ncav cuag 0.1 pg ntawm tag nrho RNA lossis tsawg dua 10 daim qauv ntawm RNA templates. thiab muaj ntau dua thermal stability.Ib Kauj Ruam QRT-PCR Probe Kit yog muab rau hauv Master Mix.Lub 5 × Ib Kauj Ruam Sib xyaw muaj qhov ua kom zoo tsis zoo thiab dNTP / dUTP Mix, thiab tsim nyog rau kev kuaj xyuas qhov tshwj xeeb raws li cov tshuaj fluorescence sau npe probes (xws li TaqMan).
Cov txheej txheem tshuaj tiv thaiv
Cheebtsam
| Cheebtsam | 100 rxn | 1,000 rns | 5,000 rx |
| RNase-dawb ddH2O | 2 * 1ml | 20 ml | 100 ml |
| 5 * ib kauj ruam sib xyaw | 600l ua | 6 * 1ml | 30 ml |
| Ib kauj ruam enzyme mix | 150l ua | 2 * 750 hli | 7.5ml ib |
| 50 * ROX siv Dye 1 | 60l ua | 600l ua | 3 * 1ml |
| 50 * ROX siv Dye 2 | 60l ua | 600l ua | 3 * 1ml |
a.Ib-kauj ruam tsis muaj xws li dNTP Mix thiab Mg2+.
b.Enzyme Mix feem ntau muaj rov qab
transcriptase, Kub Start Taq DNA polymerase (antibody modification) thiab RNase inhibitor.
c.Siv los kho qhov yuam kev ntawm fluorescene sinals ntawm cov qhov dej sib txawv.
c.ROX: Koj yuav tsum xaiv qhov ntsuas ntsuas raws li tus qauv ntawm cov ntsuas ntsuas.
Daim ntawv thov
Kev txheeb xyuas cov kab mob
diagnostics thiab kev tshawb fawb ntawm qog
Kev kuaj tsiaj txhu
Kev kuaj mob ntxov ntawm cov kab mob hereditary
Kev kuaj xyuas cov kab mob pathogenic hauv cov zaub mov
Shipping thiab Cia
Kev thauj mus los:Cov hnab ntim dej khov
Kev cia khoom:Khaws ntawm -30 ~ -15 ℃.
Shief lub neej:1 xyoo
