Ib Kauj Ruam RT-qPCR SYBR Green Kit

Universal Blue qPCR Master Mix (Dye Based) yog ib qho kev daws teeb meem ua ntej rau 2 × real-time quantitative PCR amplification yam ntxwv los ntawm siab rhiab heev thiab tshwj xeeb, yog xiav xim, thiab muaj cov nyhuv ntawm cov qauv ntxiv tracing.Cov ntsiab lus tseem ceeb Taq DNA polymerase siv cov tshuaj tiv thaiv kab mob kub pib ua kom muaj txiaj ntsig zoo inhibit qhov tsis muaj kev nthuav dav vim yog primer annealing thaum cov qauv npaj.Nyob rau tib lub sijhawm, cov qauv no ntxiv cov kev txhawb nqa los txhim kho qhov kev ua tau zoo ntawm PCR cov tshuaj tiv thaiv thiab sib npaug ntawm cov noob caj noob ces nrog cov ntsiab lus GC sib txawv (30 ~ 70%), kom ntau PCR tuaj yeem tau txais kev sib raug zoo linear hauv ntau qhov ntau. cheeb tsam.Cov khoom no muaj tshwj xeeb ROX Passive Reference Dye, uas muaj feem xyuam rau tag nrho cov twj qPCR.Nws tsis yog tsim nyog los kho qhov concentration ntawm ROX ntawm cov twj paj nruag sib txawv.Nws tsuas yog tsim nyog ntxiv primers thiab templates los npaj cov tshuaj tiv thaiv rau kev nthuav dav.