Ib Kauj Ruam RT-qPCR cov khoom siv sojntsuam
Kev piav qhia
U + Ib Kauj Ruam RT-qPCR Cov Khoom Siv Tshuaj Ntsuam Xyuas (Glycerol-dawb) yog glycerol-dawb ib-kauj ruam RT-qPCR reagent siv RNA ua tus qauv (xws li RNA tus kab mob), uas tsim nyog rau kev txhim kho thiab tsim cov khoom lyophilized.Cov khoom no sib xyaw ua ke cov kev ua tau zoo tshaj plaws ntawm ib-kauj ruam kev mob siab rau Reverse Transcriptase thiab kub-pib Champagne Taq DNA Polymerase, nrog zoo-optimized freeze-drying tshuaj tsis zoo, uas muaj kev ua kom zoo dua amplification, sib npaug thiab tshwj xeeb, thiab yog sib xws nrog sib txawv khov-drying. cov txheej txheem.Ntxiv mus, dUTP / UDG tiv thaiv kev ua qias tuaj rau hauv cov reagent, uas tuaj yeem ua haujlwm ntawm chav tsev kub, tshem tawm qhov cuam tshuam ntawm kev nthuav dav cov khoom sib kis ntawm qPCR, thiab xyuas kom meej qhov tseeb ntawm cov txiaj ntsig.
Basic Principles ntawm RT-qPCR
Specification
| Cov khoom kuaj | Specification | Kev tshwm sim |
| (SDS PAGE) Purity of Enzyme Stock (SDS PAGE) | ≥95% | Hla |
| Endonuclease Activity | Tsis pom | Hla |
| Kev ua Exodulease | Tsis pom | Hla |
| Rnase Activity | Tsis pom | Hla |
| Residual E.coli DNA | 1copies/60 | Hla |
| Functional Assay-System | 90% ≤ 110% | Hla |
Cheebtsam
| Cheebtsam | 100 rxn | 1,000 rns | 5,000 rx |
| RNase-dawb ddH2O | 2 * 1ml | 20 ml | 100 ml |
| 5 * ib kauj ruam sib xyaw | 600l ua | 6 * 1ml | 30 ml |
| Ib kauj ruam enzyme mix | 150l ua | 2 * 750 hli | 7.5ml ib |
| 50 * ROX siv Dye 1 | 60l ua | 600l ua | 3 * 1ml |
| 50 * ROX siv Dye 2 | 60l ua | 600l ua | 3 * 1ml |
a.Ib-kauj ruam tsis muaj xws li dNTP Mix thiab Mg2+.
b.Enzyme Mix feem ntau muaj rov qab
transcriptase, Kub Start Taq DNA polymerase (antibody modification) thiab RNase inhibitor.
c.Siv los kho qhov yuam kev ntawm fluorescene sinals ntawm cov qhov dej sib txawv.
c.ROX: Koj yuav tsum xaiv qhov ntsuas ntsuas raws li tus qauv ntawm cov ntsuas ntsuas.
Daim ntawv thov
QPCR nrhiav pom
Shipping thiab Cia
Kev thauj mus los:Cov hnab ntim dej khov
Kev cia khoom:Khaws ntawm -20 ℃.
Shief Lub Neej:18 hli
