Bst 2.0 DNA Polymerase (Glycerol dawb, siab ceev)
Bst DNA polymerase V2 yog muab los ntawm Bacillus stearothermophilus DNA Polymerase I, uas muaj 5′ → 3′ DNA polymerase kev ua thiab muaj zog saw hloov ua haujlwm, tab sis tsis muaj 5′→ 3′ exonuclease kev ua.Bst DNA Polymerase V2 yog qhov tsim nyog rau strand-hloov chaw, isothermal amplification LAMP (Loop mediated isothermal amplification) thiab ceev sequencing.Qhov no Bst DNA polymerase V2 muaj peev xwm ntawm inhibiting DNA polymerase kev ua haujlwm ntawm chav tsev kub, kom nws tuaj yeem ua haujlwm thiab cov txheej txheem tshuaj tiv thaiv tuaj yeem tsim nyob rau hauv chav tsev kub, tiv thaiv tsis muaj kev nthuav dav thiab txhim kho kev ua haujlwm ntawm cov tshuaj tiv thaiv, thiab cov qauv no tuaj yeem ua tau. yuav lyophilized.Tsis tas li ntawd, nws muaj peev xwm tso tawm nws cov haujlwm ntawm qhov kub siab, yog li tshem tawm qhov xav tau rau kev ua kom cov kauj ruam cais.
Cheebtsam
| Cheebtsam | TSI HC5007A-01 | TSI HC5007A-02 | TSI HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-dawb) (32U / μL) | 0.05 ml ib | 0,25 ml yog ' | 2.5 ml ib |
| 10 × HC Bst V2 Buffer | 1.5 ml yog ' | 2 × 1.5 mL | 3 × 10 ml |
| MgSO4 (100 hli) | 1.5 ml yog ' | 2 × 1.5 mL | 2 × 10 ml |
Daim ntawv thov
1.LAMP isothermal amplification
2.DNA strand ib qho kev hloov pauv hloov
3.High GC gene sequencing
4.DNA sequencing ntawm nanogram theem.
Kev cia khoom
Kev thauj mus los hauv qab 0 ° C thiab khaws cia ntawm -25 ° C ~ -15 ° C.
Unit txhais
Ib chav tsev tau txhais tias yog tus nqi ntawm cov enzyme uas suav nrog 25 nmol ntawm dNTP rau hauv cov khoom siv acid insoluble hauv 30 feeb ntawm 65 ° C.
LAMP Reaction
| Cheebtsam | 25 μLQhov system |
| 10 × HC Bst V2 Buffer | 2.5 l ua |
| MgSO4 (100 hli) | 1.5 l ua |
| dNTPs (10mM txhua) | 3,5l ua |
| SYTO™ 16 Green (25 ×)a | 1.0 l os |
| primer mixb | 6l miv |
| Bst DNA Polymerase V2 (Glycerol-dawb) (32 U/uL) | 0.25 mL ib |
| Template | × μL |
| ddH₂O | Txog li 25 μL |
Nco tseg:
1) a.SYTOTM 16 Green (25 ×): Raws li kev sim xav tau, lwm yam xim tuaj yeem siv los hloov pauv;
2) ib.Primer mix: tau los ntawm kev sib xyaw 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB thiab lwm yam ntim.
Cov tshuaj tiv thaiv thiab mob
1 × HC Bst V2 Buffer, qhov ntsuas kub ntawm 60 ° C thiab 65 ° C.
Kub Inactivation
80 ° C, 20 mins.
