Qauv tso tawm Reagent
Qauv Tso Tawm Reagent yog rau molecular POCT kuaj mob scenarios.Rau ob lub tshuab hluav taws xob hluav taws xob hluav taws xob thiab hluav taws xob hluav taws xob ncaj qha PCR, tsis tas yuav muaj cov nucleic acid extraction.Lub crude lysate ntawm cov qauv tuaj yeem raug nthuav dav ncaj qha, lub hom phiaj ntawm cov noob tuaj yeem raug kuaj pom tseeb, lub sijhawm kuaj xyuas cov qauv tuaj yeem ua kom luv ntxiv, uas zoo sib xws rau cov ntawv thov ntawm molecular POCT.Nws yog haum rau qhov ntswg swabs, caj pas swabs thiab lwm yam qauv.Cov qauv ua tiav tuaj yeem siv ncaj qha rau lub sijhawm tiag tiag fluorescence quantitative PCR lossis LAMP nrhiav pom, thiab cov txiaj ntsig zoo ib yam li cov txheej txheem rho tawm tuaj yeem ua tiav yam tsis muaj kev ua haujlwm nyuaj nucleic acid extraction.
Kev cia khoom
Kev thauj mus los thiab khaws cia ntawm chav tsev kub.
Kev tswj kom zoo
Kev kuaj pom muaj nuj nqi - ntau qPCR: 800μl Cov Qauv Tso Cai Reagent tau nthuav dav
nrog 1000 daim ntawv luam tawm Novel Pseudovirus, ib qho qauv ntawm qhov ntswg qhov ntswg, ua rau muaj qhov sib npaug zoo sib xws thiabΔCt qhov tseem ceeb hauv ± 0.5 Ct.
Experimental Procedures
1. Siv 800 μl Qauv Tso Cai Reagent thiab kis cov tshuaj lysis rau hauv 1.5 mL sampling raj
2. Siv lub qhov ntswg los yog lub caj pas swab nrog lub swab; Nasal swab sampling txheej txheem: nqa cov sterile swab thiab muab tso rau hauv lub qhov ntswg, maj mam nce mus txog 1.5 cm tob, maj mam tig 4 zaug tiv thaiv qhov ntswg mucosa rau ntau tshaj 15 vib nas this. , tom qab ntawd rov ua haujlwm tib yam ntawm lwm lub qhov ntswg nrog tib lub swab. caj pas swab sampling txheej txheem: nqa lub sterile swab thiab maj mam, sai sai so lub pharyngeal tonsils thiab rear pharyngeal phab ntsa 3 zaug.
3.Muab lub swab tam sim ntawd tso rau hauv lub raj coj mus kuaj.Lub taub hau swab yuav tsum tau tig thiab sib tov rau hauv qhov kev daws teeb meem tsawg kawg 30 vib nas this kom paub meej tias cov qauv yog tag nrho eluted hauv cov qauv raj.
4. Incubation ntawm chav tsev kub (20 ~ 25 ℃) rau 1min, kev npaj ntawm lysis buffer tiav.
5. Ob leeg 25μl system RT-PCR thiab RT-LAMP tau sib xws nrog 10μl tus nqi ntawm cov qauv ntxiv rau kev kuaj pom.
Sau ntawv
1. Qhov tsawg kawg nkaus ntawm cov qauv ncaj qha lysate sib haum rau ib qho swab tuaj yeem hloov kho rau 400μl, uas tuaj yeem hloov kho raws li qhov xav tau ntawm kev sim.
2. Thaum cov qauv ua tiav los ntawm cov qauv tso tawm reagent, nws raug nquahu kom ua cov theem tom ntej ntawm kev sim sai li sai tau, lub sijhawm tos lub sijhawm yog nyiam tsawg dua 1 teev.
3. Cov pH ntawm cov qauv lysate yog acidic, thiab lub tshuab kuaj xyuas yuav tsum muaj qee yam tsis muaj.Nws yog haum rau feem ntau PCR, RT-PCR, thiab LAMP fluorescence nrhiav kom tau nrog pH tsis, tab sis tsis haum rau LAMP colorimetric nrhiav tsis tau.
