Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase yog kub pib DNA polymerase nrog ob chav thaiv los ntawm ob chav tshuaj tiv thaiv kab mob.Cov khoom no tsis tsuas yog thaiv cov 5′ → 3′ polymerase kev ua ntawm Taq DNA polymerase, tab sis kuj thaiv cov 5′→ 3′ exonuclease kev ua.Cov cua sov rau 30 vib nas this ntawm qhov kub ua ntej denaturation tuaj yeem ua rau cov tshuaj tiv thaiv kab mob thiab tso tawm DNA polymerase kev ua haujlwm thiab kev ua haujlwm exonuclease.Ob chav thaiv cov yam ntxwv tuaj yeem tsis tsuas yog tiv thaiv qhov tsis zoo ntawm kev nthuav dav los ntawm kev tsis sib haum xeeb lossis primer dimer, tab sis kuj tseem cuam tshuam qhov poob ntawm cov teeb liab fluorescence tshwm sim los ntawm kev sojntsuam degradation, thiaj li ua kom lub hauv vitro nrhiav kom pom reagent ruaj khov thaum thauj lossis siv hauv chav. kub.
Cheebtsam
| Cheebtsam | HC10 12B (250 U) | HC10 12B (1000U) | HC10 12B (10000U) | HC10 12B (25000U) |
| Cov DNA Polymerase(5 U / μL) | 50l ua | 200 mL | 2 ml ib | 5 ml ib |
Kev cia khoom
Cov khoom xa tuaj nrog cov dej khov qhuav thiab tuaj yeem khaws cia ntawm -25 ° C ~ -15 ° C rau 2 xyoos.
Specifications
| Polymerase | Cov DNA Polymerase |
| Purity | ≥ 95% (SDS-PAGE) |
| Pib kub | Built-in Kub Pib |
| Teb ceev | Txuj |
| Kev Ua Haujlwm Exonuclease | 5′ → 3′ |
Cov lus qhia
Kev teeb tsa kev daws teeb meem
| Cheebtsam | Ntim (μL) | Thaum kawg Concentration |
| 2 × Buffera | 25 | 1 × |
| Primer/Probe mixb | × | 0.1 μmol / L - 0.5 μmol / L |
| Hotstart Taq Polymerase (5U / μL) | 1.2 | 0.12 U / μL |
| DNA Templatec | × | 0.1-100 hli |
| ddH ua2O | Txog li 50 | - |
Nco tseg:
1) Raws li daim ntawv thov kev sim tshwj xeeb, nws yuav tsum tau npaj cov tshuaj tiv thaiv tsis sib xws.
2) Tus nqi ntawm DNA thiab cov concentration ntawm kev sojntsuam los yog primers yog pom zoo kom muaj concentrations.Qhov zoo tshaj plaws concentration tuaj yeem hloov kho raws li qhov kev sim tshwj xeeb.
Thermal cycling raws tu qauv
| Kauj ruam | Kub(°C) | Sijhawm | Lub voj voog |
| Pre-denaturation | 95 ℃ | 5 feeb | 1 |
| Denaturation | 95 ℃ | 15 sec | 45 |
| Annealing / Extension | 60 ℃ib | 30 secb |
Nco tseg:
1) Cov tshuaj tiv thaiv kub yog hloov raws li tus nqi Tm ntawm cov tsim primers.
2) Cov cuab yeej sib txawv qPCR xav tau lub sijhawm sib txawv ntawm cov teeb liab fluorescence, thov teem caij raws li lub sijhawm luv tshaj.
Sau ntawv
Thov hnav PPE tsim nyog, xws li lub tsho tiv no thiab hnab looj tes, txhawm rau ua kom koj noj qab haus huv thiab nyab xeeb!
