Robustart Taq DNA Polymerase

Robustart Taq DNA Polymerase yog qhov pib kub pib DNA polymerase.Cov khoom no tuaj yeem tsis tsuas yog zoo dua inhibit qhov tsis muaj cov tshuaj tiv thaiv tshwj xeeb los ntawm qhov tsis yog tshwj xeeb annealing ntawm primers los yog primer aggregation nyob rau hauv tus txheej txheem ntawm PCR system npaj thiab amplification.Yog li ntawd, nws muaj qhov tshwj xeeb zoo heev thiab muaj txiaj ntsig zoo rau kev nthuav dav ntawm cov qauv uas tsis tshua muaj siab, thiab nws tsim nyog rau cov tshuaj tiv thaiv multixed PCR amplification.Ntxiv mus, cov khoom no muaj kev siv tau zoo heev, thiab cov txiaj ntsig kev ua kom muaj zog ruaj khov tuaj yeem tau txais hauv ntau hom PCR cov tshuaj tiv thaiv.

Cheebtsam

1.5 U/μL Robusstart Taq DNA polymerase

2.10 × PCR Buffer II (Mg² + dawb) (yeem)

3.25 mMgcl₂(yeem)

* 10 × PCR Buffer II (Mg²+ pub dawb) tsis muaj dNTP thiab Mg²+, thov ntxiv dNTPs thiab MgCl₂thaum npaj cov tshuaj tiv thaiv kab mob.

Cov ntawv thov pom zoo

1.Ceev amplification.

2.Multiple amplification.

3.Direct amplification ntawm cov ntshav, swabs, thiab lwm yam qauv.

4.Kev kuaj mob ua pa.

Kev cia khoom

-20 ° C rau lub sij hawm ntev cia, yuav tsum tau tov zoo ua ntej siv, tsis txhob nquag khov-thaw.

* Yog tias nag lossis daus tshwm sim tom qab lub tub yees, nws yog qhov qub;nws raug pom zoo kom sib npaug rau chav tsev kub ua ntej sib tov thiab siv.

Unit txhais

Ib chav ua haujlwm (U) txhais tau tias yog tus nqi ntawm cov enzyme uas suav nrog 10 nmol ntawm deoxyribonucleotide rau hauv cov khoom siv acid-insoluble ntawm 74 ° C rau 30mins siv cov activated salmon phev DNA ua qauv / primer.

Kev Tswj Xyuas Zoo

1.SDS-PAGE electrophoretic purity ntau dua 98%.

2.Amplification rhiab heev, batch-to-batch tswj, stability.

3.Tsis muaj exogenous nuclease kev ua, tsis muaj exogenous endonuclease los yog exonuclease paug

Cov lus qhia

Kev teeb tsa kev daws teeb meem

Nco tseg:

1) a.Qhov tsis muaj dNTP thiab Mg² +, thov ntxiv dNTPs thiab MgCl₂thaum npaj cov tshuaj tiv thaiv kab mob.

2) ib.Yog siv rau qPCR/qRT-PCR, fluorescent probes yuav tsum tau ntxiv rau cov tshuaj tiv thaiv kab mob.Feem ntau, qhov kawg primer concentration ntawm 0.2 μM yuav muab tau zoo;Yog tias cov tshuaj tiv thaiv kev ua haujlwm tsis zoo, cov primer concentration tuaj yeem hloov kho hauv thaj tsam ntawm 0.2-1 μM.Kev sojntsuam concentration feem ntau yog optimized nyob rau hauv thaj tsam ntawm 0.1-0.3 μM.Concentration gradient thwmsim tuaj yeem ua tau los nrhiav qhov zoo tshaj plaws ua ke ntawm primer thiab sojntsuam.

Thermal cycling raws tu qauv

Sau ntawv

1.Lub amplification tus nqi ntawm ceev DNA polymerase yuav tsum tsis txhob tsawg tshaj li 1 kb / 10 s.Qhov kub nce thiab poob qis, kev tswj qhov kub thiab txias ua haujlwm ntawm cov cuab yeej PCR sib txawv heev, yog li nws raug pom zoo kom ua kom zoo dua cov tshuaj tiv thaiv zoo rau cov cuab yeej PCR ceev.

2.Lub system yog adaptable heev, nrog ntau dua specification thiab rhiab heev.

3.Haum rau siv raws li siab rhiab heev PCR nrhiav reagents, thiab yuav siv tau nyob rau hauv multiplex PCR amplification tshwm sim.

4.5′ → 3 ′ polymerase kev ua, 5′ → 3′ exonuclease kev ua;tsis muaj 3′→ 5′ exonuclease kev ua;tsis muaj proofreading function.

5.Tsim nyog rau kev kuaj kom zoo thiab ntau ntawm PCR thiab RT-PCR.

6.Qhov 3' kawg ntawm cov khoom PCR yog A, uas tuaj yeem ncaj qha cloned rau hauv T vector.

7.Txoj kev peb-kauj ruam yog pom zoo rau primers uas tsis tshua muaj annealing kub los yog rau amplification ntawm fragments ntev tshaj 200 bp.