Poly A Carrier RNA

Cat No: HC4001A

Poly A, polyadenylate, yog sib xyaw ntawm 100 ~ 10000 polyadenylates, uas yog polymerized los ntawm polynucleotide phosphorylase hauv vitro.Hauv vivo, poly (a) yog ntxiv rau 3-terminal ntawm mRNA los ntawm enzyme los txhim kho kev ruaj ntseg ntawm mRNA.Nyob rau hauv daim ntawv thov ntawm nucleic acid extraction, ntxiv poly A rau lysate los yog binding tov tuaj yeem txhim kho cov txiaj ntsig ntawm DNA thiab RNA.Lub mechanismntawm poly A txhim kho cov txiaj ntsig ntawm nucleic acid yog raws li hauv qab no:

1. Saturated tiv tauj nrog rau saum npoo adsorption ntawm cov khoom.Feem ntau cov khoom siv polypropylene muaj hluav taws xob zoo li qub rau ntawm qhov chaw, uas yuav adsorb nucleic acids.Carrier RNA tuaj yeem saturate cov noadsorption cuam tshuam thiab txo qhov poob ntawm lub hom phiaj nucleic acids.

2. Inactivate trace nucleases: Muaj ntau yam nucleases hauv cov qauv lom neeg thiabib puag ncig.Poly A tuaj yeem cuam tshuam cov kab nucleases hauv kev rho tawm lossis khaws cia rautxhim kho cov txiaj ntsig thiab kev ruaj ntseg ntawm lub hom phiaj nucleic acids.

3. Coprecipitation: Nyob rau hauv lub nucleic acid purification kauj ruam ntawm cawv mediated nag lossis khi, poly A tuaj yeem coprecipitate nrog lub hom phiaj nucleic acid los yog tsim cov khoom polymer rautxhim kho kev rov zoo.

Txoj kev siv

Siv cov hmoov lyophilized, ntxiv DEPC dej kho los yog guanidine ntsev tov rauyaj nws mus rau hauv 0.1-1ug / uL, thiab ces sub-pack nws thiab khaws cia ntawm -20 ° C.

Daim ntawv thov

1.Virus DNA / RNA rho tawm: ntxiv 1-5ug Carrier RNA rau lysate tuaj yeem txhim kho cov txiaj ntsig ntawm RNA / DNA, stabilize lub hom phiaj nucleic acid thiab tsis txhob degradation ntawm purified nucleic acid thaum lub sij hawm cia.

2. Nyob rau hauv micro DNA/RNA rho tawm los ntawm kab membrane txoj kev (<1ug), ntxiv cov cab kuj RNA rau 1-5ug yog conducive los txhim kho cov yield ntawm nucleic acid.

3. Nyob rau hauv cov dej cawv mediated nucleic acid nag lossis daus thiab concentration theem, qhov sib ntxiv ntawm 1-2ug cab kuj RNA yog pab los txhim kho lub rov qab ntawm luv ntu RNA.

4. Nyob rau hauv qhov kev sojntsuam ntau PCR cov tshuaj tiv thaiv, ntxiv 10-100ng cab cab RNA rau cov tshuaj tiv thaiv yog pab txhim kho qhov rhiab heev thiab txo qhov C._Ttus nqi.