Ultra Nuclease GMP-qib
Cat No: HC2016A
UltraNuclease GMP-qib qhia thiab ua kom huv hauv Escherichia coli (E.coli) los ntawm kev tsim kho caj ces thiab npaj raws li GMP ib puag ncig.Nws tuaj yeem txo cov viscosity ntawm cell supernatant thiab cell lysate hauv kev tshawb fawb tshawb fawb, ua kom cov protein purification efficiency thiab txhim kho cov kev tshawb fawb ntawm cov protein.Cov khoom lag luam tseem tuaj yeem txo tus tswv tsev nucleic acid residues rau pg-qib, txhim kho kev ua haujlwm thiab kev nyab xeeb ntawm cov khoom siv roj ntsha ntawm cov ntawv thov suav nrog kev ua kom huv ntawm tus kab mob, kev tsim tshuaj tiv thaiv, thiab kev tsim tshuaj protein / polysaccharide.Tsis tas li ntawd, cov khoom lag luam tseem tuaj yeem siv los tiv thaiv cov kab mob ntawm tib neeg peripheral ntshav mononuclear hlwb (PBMC) hauv kev kho ntawm tes thiab kev tsim tshuaj tiv thaiv.
UltraNuclease yog muab nyob rau hauv daim ntawv ntawm sterilized reagent, eluted nyob rau hauv buffer (20mM Tris-HCL pH 8.0, 2mM MgCl, 20 mM NaCl, 50% glycerin), nrog rau cov tsos ntawm ib tug tsis muaj kob, pob tshab kua.Cov khoom no yog tsim los ntawm cov txheej txheem GMP thiab muab rau hauv daim ntawv ua kua.
Cheebtsam
UltraNuclease GMP-qib (250 U / μL)
Kev cia khoom
Cov khoom xa tuaj nrog cov dej khov qhuav thiab tuaj yeem khaws cia ntawm -25 ℃ ~ -15 ℃ rau ob xyoos.
Yog tias cov khoom tau qhib thiab tau khaws cia ntawm 4 ℃ rau ntau tshaj ib lub lim tiam, peb pom zoo kom limcov khoom tiv thaiv kab mob microbial.
Specifications
| Expression Host | Recombinant E. coli nrog UltraNuclease noob |
| Molecular Luj | 26,5kd ua |
| soelectric point | 6.85 ib |
| Purity | ≥99% (SDS-PAGE) |
| Storage Buffer | 20mM Tris-HCL pH 8.0, 2mM MgCl, 20 mM NaCl, 50% glycerin |
|
Unit txhais | Lub ntsiab txhais ntawm ib qho Kev Ua Haujlwm (U) yog tus nqi ntawm cov enzyme siv loshloov tus nqi nqus ntawm ΔA260 los ntawm 1.0 hauv 30 feeb hauv 2.625 mLCov tshuaj tiv thaiv kab mob ntawm 37 ℃ nrog pH ntawm 8.0 (sib npaug ua kom tiav kev zom zaub mov37 μg salmon phev DNA rau hauv oligonucleotides). |
Cov lus qhia
1. Qauv Sau
Adherent cells: tshem tawm qhov nruab nrab, ntxuav lub hlwb nrog PBS, thiab tshem tawm cov supernatant.
Tshem tawm cov hlwb: sau cov hlwb los ntawm centrifugation, ntxuav cov hlwb nrog PBS, centrifuge ntawm 6,000rpm rau 10 min, sau cov pellet.
Escherichia coli: sau cov kab mob los ntawm centrifugation, ntxuav ib zaug nrog PBS, centrifuge ntawm 8,000rpm rau 5 min, thiab sau cov pellet.
2. Qauv kho
Kho cov cell pellets uas tau sau nrog lysis tsis nyob ntawm qhov sib piv ntawm qhov loj (g) rau ntim (mL) 1: (10-20), lossis los ntawm kev siv tshuab lossis tshuaj rau ntawm cov dej khov lossis hauv chav tsev kub (1g ntawm cov cell pellet muaj txog
109 cev).
3. Kev kho enzyme
Ntxiv 1-5mM MgCl rau cov tshuaj tiv thaiv kab mob thiab kho cov pH rau 8-9.
Ntxiv UltraNuclease raws li qhov piv ntawm 250 units rau zom 1 g ntawm cell pellets, incubate ntawm 37 ℃ rau ntau tshaj 30 feeb.Thov xa mus rau daim ntawv "Recommended Reaction Time" los xaiv qhovntev ntawm kev kho mob.
4. Supernatant Sau
Centrifuge ntawm 12,000 rpm rau 30 feeb thiab sau cov supernatant.
Lus Cim: Yog hais tias cov tshuaj yog acidic los yog alkaline, los yog muaj siab concentrations ntawm ntsev, detergents, los yogdenaturants, thov nce enzyme ntau npaum li cas los yog ncua sij hawm kho raws li.
Pom zoo cov tshuaj tiv thaivons
| Parameter | Qhov zoo tshaj plaws | Cov yam ntxwv zoo |
| Mg² + Concentration | 1-5 mM | 1-10 mM |
| pH | 8-9 | 6-10 |
| Kub | 37 ℃ | 0-42 ℃ |
| DTT Concentration | 0-100 hli | > 0 mM |
| Mercaptoethanol Concentration | 0-100 hli | > 0 mM |
| Monovalent Cation Concentration | 0-20 hli | 0-150 hli |
| Phosphate lon Concentration | 0-10 hli | 0-100 hli |
Pom zoo Cov tshuaj tiv thaiv Sijhawm (37 ℃, 2 mM Mg²+, pH 8.0)
| Tus nqi ntawm UltraNuclease (Thaum kawg Concentration) | Lub Sijhawm Tawm Tsam |
| 0.25 U / ml | > 10 h |
| 2.5 U / ml | > 4 h |
| 25 U / ml | 30 feeb |
Nco tseg:
Thov hnav PPE tsim nyog, xws li lub tsho tiv no thiab hnab looj tes, txhawm rau ua kom koj noj qab haus huv thiab nyab xeeb!
