Viral DNA/RNA Extraction Kit
Cov khoom siv no tsim nyog rau kev tshem tawm sai ntawm high-purity viral DNA / RNA los ntawm cov qauv xws li nasopharyngeal swabs, ib puag ncig swabs, cell culture supernatants, thiab cov ntaub so ntswg homogenate supernatants.Cov khoom siv yog ua raws li silica membrane purification technology uas tshem tawm qhov xav tau ntawm kev siv phenol / chloroform organic solvents los yog haus dej cawv los ntawm lub sij hawm los rho cov kab mob DNA / RNA ntawm cov khoom zoo.Cov nucleic acids tau txais yog dawb ntawm impurities thiab npaj rau siv nyob rau hauv downstream thwmsim xws li rov qab transcription, PCR, RT-PCR, real-time PCR, tiam tom ntej sequencing (NGS), thiab Northern blot.
Cia tej yam kev mob
Khaws ntawm 15 ~ 25 ℃, thiab thauj hauv chav tsev kub
Cheebtsam
| Cheebtsam | 100 RXNS |
| Buffer VL | 50 ml ib |
| Buffer RW | 120 ml |
| RNase-dawb ddH2 O | 6ml ib |
| FastPure RNA Kab | 100 |
| Collection Tubes (2ml) | 100 |
| RNase-dawb Collection Tubes (1.5ml) | 100 |
Buffer VL:Muab ib puag ncig rau lysis thiab khi.
Buffer RW:Tshem tawm cov protein seem thiab lwm yam impurities.
RNase-dawb ddH2O:Elute DNA / RNA los ntawm daim nyias nyias hauv kab rov tav.
FastPure RNA Kab:Tshwj xeeb adsorb DNA / RNA.
Collection Tubes 2 ml:Sau cov filtrate.
RNase-dawb Collection Tubes 1.5 ml:Sau DNA/RNA.
Daim ntawv thov
Nasopharyngeal swabs, ib puag ncig swabs, cell culture supernatants, thiab cov ntaub so ntswg homogenate supernatants.
Tus kheej npaj Materials
RNase-free pipette, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, thiab pipettes.
Kev sim txheej txheem
Ua tag nrho cov kauj ruam hauv qab no hauv lub txee biosafety.
1. Ntxiv 200 μl ntawm cov qauv mus rau RNase-free centrifuge raj (ua nrog PBS lossis 0.9% NaCl hauv cov qauv tsis txaus), ntxiv 500 μl ntawm Buffer VL, sib tov zoo los ntawm vortexing rau 15 - 30 sec, thiab centrifuge luv luv kom sau cov sib tov rau hauv qab ntawm lub raj.
2. Muab FastPure RNA Kem rau hauv Cov Tubes Sau 2 ml.Hloov cov sib tov los ntawm Kauj Ruam 1 mus rau FastPure RNA Kab, centrifuge ntawm 12,000 rpm (13,400 × g) rau 1 min, thiab muab pov tseg cov lim.
3. Ntxiv 600 μl ntawm Buffer RW rau FastPure RNA Kab, centrifuge ntawm 12,000 rpm (13,400 × g) rau 30 sec, thiab muab pov tseg cov lim.
4. Rov ua kauj ruam 3.
5. Centrifuge lub khoob khoob ntawm 12,000 rpm (13,400 × g) rau 2 min.
6. Ua tib zoo hloov FastPure RNA Kab rau hauv RNase-free Collection Tubes 1.5 ml (muab hauv cov khoom siv), thiab ntxiv 30 - 50 μl ntawm RNase-dawb ddH2O rau hauv nruab nrab ntawm daim nyias nyias yam tsis txhob kov cov kem.Cia sawv ntsug ntawm chav tsev kub rau 1 min thiab centrifuge ntawm 12,000 rpm (13,400 × g) rau 1 min.
7. Tshem tawm FastPure RNA kab.DNA / RNA tuaj yeem siv ncaj qha rau kev soj ntsuam tom ntej, lossis khaws cia ntawm -30 ~ -15 ° C rau lub sijhawm luv lossis -85 ~ -65 ° C rau lub sijhawm ntev dua.
Sau ntawv
Rau kev tshawb fawb siv xwb.Tsis siv rau hauv cov txheej txheem kuaj mob.
1. Equilibrate cov qauv mus rau chav tsev kub ua ntej.
2. Kab mob kis tau zoo heev.Thov xyuas kom tag nrho cov kev tiv thaiv kev nyab xeeb tsim nyog tau ua ua ntej kev sim.
3. Tsis txhob rov khov thiab thawing ntawm cov qauv, vim qhov no yuav ua rau degradation los yog txo cov txiaj ntsig ntawm rho tawm cov kab mob DNA/RNA.
4. Cov cuab yeej npaj rau tus kheej muaj xws li RNase-free pipette cov lus qhia, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex tov khoom, thiab pipettes.
5. Thaum siv cov khoom siv, hnav lub tsho (lab), cov hnab looj tes uas siv tau, thiab daim npog ntsej muag pov tseg thiab siv cov khoom siv tsis muaj RNase los txo qhov kev pheej hmoo ntawm kev kis kab mob RNase.
6. Ua txhua kauj ruam ntawm chav tsev kub tshwj tsis yog tias tau teev tseg.
Mechanism & Workflow
