Tus kab mob DNA/RNA Extraction Kit
Cov khoom siv (HC1009B) tuaj yeem sai sai tshem tawm high-purity viral nucleic acids (DNA / RNA) los ntawm ntau cov qauv ua kua xws li ntshav, ntshav, ntshav, thiab swab ntxuav kua, ua kom muaj kev ua haujlwm siab ntawm cov qauv sib npaug.Cov khoom siv tshwj xeeb embedded superparamagnetic silicon-based magnetic hlaws.Nyob rau hauv ib qho kev tsis sib xws, nucleic acids es tsis txhob ntawm cov proteins thiab lwm yam impurities yog adsorbed los ntawm hydrogen bonds thiab electrostatic binding.Cov hlaws sib nqus uas muaj adsorbed nucleic acids yog ntxuav kom tshem tawm cov protein thiab ntsev ntxiv.Thaum siv cov ntsev tsis tshua muaj ntsev, cov nucleic acids raug tso tawm los ntawm cov hlaws sib nqus, thiaj li ua tiav lub hom phiaj ntawm kev sib cais sai thiab ua kom huv ntawm nucleic acids.Tag nrho cov txheej txheem ua haujlwm yog yooj yim, ceev, nyab xeeb thiab ua haujlwm tau zoo, thiab cov nucleic acids tau siv ncaj qha rau kev sim qis qis xws li thim rov qab, PCR, qPCR, RT-PCR, RT-qPCR, tiam tom ntej sequencing, biochip tsom xam, lwm.
Cia tej yam kev mob
Khaws ntawm 15 ~ 25 ℃, thiab thauj hauv chav tsev kub.
Daim ntawv thov
Ntshav, ntshav, ntshav ntshav, swab eluent, ntaub so ntswg homogenate thiab lwm yam.
Kev sim txheej txheem
1. Qauv ua
1.1 Rau cov kab mob hauv cov qauv ua kua xws li ntshav, ntshav, thiab ntshav: 300μL ntawm supernatant siv rau kev rho tawm.
2.2 Rau cov qauv swab: Muab cov qauv swab tso rau hauv cov qauv coj mus kuaj uas muaj cov tshuaj khaws cia, vortex rau 1 min, thiab siv 300μL supernatant rau extraction.
1.3 Rau cov kab mob hauv cov ntaub so ntswg homogenates, cov ntaub so ntswg soak, thiab cov qauv ib puag ncig: sawv cev rau 5 -10 min, thiab noj 300μL ntawm supernatant rau extraction.
2. Kev npaj ntawm npajtshuaj reagent
Tshem tawm cov khoom ntim ua ntej los ntawm cov khoom siv, rov ua dua thiab sib tov ob peb zaug kom rov ua dua cov hlaws sib nqus.Maj mam co lub phaj kom cov reagents thiab magnetic hlaws dai rau hauv qab ntawm qhov dej.Thov paub meej tias cov kev taw qhia ntawm lub phaj thiab ua tib zoo rhuav tshem cov ntawv ci aluminium.
Δ Tsis txhob muaj kev vibration thaum rhuav tshem cov yeeb yaj kiab los tiv thaiv cov kua los ntawm kev nchuav.
3. Kev ua haujlwm ntawm lub automatic instrument
3.1 Ntxiv 300μL ntawm cov qauv mus rau qhov dej hauv Kab 1 lossis 7 ntawm 96 qhov sib sib zog nqus phaj phaj (ua tib zoo saib xyuas qhov ua haujlwm zoo).Cov tswv yim ntim ntawm cov qauv yog sib xws nrog 100-400 μL.
3.2 Muab 96-zoo sib sib zog nqus phaj rau hauv cov nucleic acids extractor.Muab tso rau ntawm lub tes tsho hlau nplaum, thiab xyuas kom lawv tag nrho cov ntaub ntawv sib nqus.
3.3 Teem lub program raws li hauv qab no rau kev rho tawm tsis siv neeg:
3.4 Tom qab kev rho tawm, hloov cov eluent los ntawm Kab 6 los yog 12 ntawm 96 qhov sib sib zog nqus phaj phaj (ua tib zoo saib xyuas qhov chaw ua haujlwm zoo) mus rau lub raj tsis muaj Nuclease-free centrifuge.Yog tias koj tsis siv tam sim ntawd, thov khaws cov khoom ntawm -20 ℃.
Sau ntawv
Rau kev tshawb fawb siv xwb.Tsis siv rau hauv cov txheej txheem kuaj mob.
1. Cov khoom rho tawm yog DNA/RNA.Kev saib xyuas tshwj xeeb yuav tsum tau them nyiaj txhawm rau tiv thaiv kev degradation ntawm RNA los ntawm RNase thaum ua haujlwm.Cov khoom siv thiab cov qauv siv yuav tsum tau mob siab rau.Tag nrho cov raj thiab cov lus qhia pipette yuav tsum tau ua kom tsis muaj menyuam thiab DNase / RNase-dawb.Cov neeg ua haujlwm yuav tsum hnav hnab looj tes tsis muaj hmoov thiab lub qhov ncauj qhov ntswg.
2. Thov nyeem phau ntawv qhia ua tib zoo ua ntej siv, thiab ua haujlwm nruj raws li phau ntawv qhia.Kev ua piv txwv yuav tsum tau ua nyob rau hauv lub rooj ntev zaum ultra huv lossis lub txee rau kev nyab xeeb.
3. Tsis siv neeg nucleic acid extraction system yuav tsum tau tua kab mob los ntawm UV rau 30 min ua ntej thiab tom qab siv.
4. Tej zaum yuav muaj cov kab sib nqus ntawm cov hlaws dai nyob hauv qhov eluent tom qab rho tawm, yog li tsis txhob aspirating cov hlaws sib nqus.Yog tias cov hlaws sib nqus tau aspirated, nws tuaj yeem raug tshem tawm nrog lub ntsej muag sib nqus.
5. Yog tias tsis muaj cov lus qhia tshwj xeeb rau cov khoom sib txawv ntawm cov reagents, thov tsis txhob sib tov, thiab xyuas kom meej tias cov khoom siv tau siv nyob rau lub sijhawm siv tau.
6. Muab tag nrho cov qauv thiab cov reagent pov tseg kom zoo, so kom huv si thiab tshuaj tua kab mob txhua qhov chaw ua haujlwm nrog 75% ethanol.
