Peroxidase (Horseradish qhov chaw) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) yog cais los ntawm cov hauv paus hniav ntawm horseradish (Amoracia rusticana) thiab belongs rau pawg ferroprotoporphyrin ntawm peroxidases.HRP nkag tau nrog hydrogen peroxide (H2O2).Qhov tshwm sim [HRP-H2O2] complex tuaj yeem oxidize ntau yam ntawm hydrogen pub dawb:
Tus pub + H2O2 → Oxidized pub + 2 H2O
HRP yuav oxidize ntau yam substrates (saib Table 1):
• Chromogenic
• Chemiluminescent (xws li luminol lossis isoluminol)
• Fluorogenic (xws li tyramine, homovanillic acid, los yog 4-hydroxyphenyl acetic acid)
HRP yog ib qho saw hlau polypeptide uas muaj plaub qhov txuas txuas ntxiv.HRP yog glycoprotein uas muaj 18% carbohydrate.Cov carbohydrate muaj pes tsawg leeg muaj galactose, arabinose, xylose, fucose, mannose, mannosamine, thiab galactosamine, nyob ntawm seb cov isozyme tshwj xeeb.

HRP yog ib daim ntawv lo rau cov tshuaj immunoglobulins hauv ntau daim ntawv thov kev tiv thaiv kab mob sib txawv, suav nrog cov tshuaj tiv thaiv kab mob, immunohistochemistry, thiab ELISA.HRP tuaj yeem txuas rau cov tshuaj tiv thaiv los ntawm ntau txoj kev sib txawv, suav nrog glutaraldehyde, ntu oxidation, los ntawm disulfide bonds, thiab tseem ntawm amino thiab thiol qhia cross-linkers.HRP yog daim ntawv lo uas xav tau tshaj plaws rau cov tshuaj tiv thaiv kab mob, vim tias nws yog qhov tsawg tshaj plaws thiab ruaj khov ntawm peb cov npe enzyme nrov tshaj plaws (peroxidase, β-galactosidase, alkaline phosphatase) thiab nws cov glycosylation ua rau txo qis tsis muaj kev sib khi.Kev tshuaj xyuas ntawm glutaraldehyde thiab periodate conjugation txoj kev tau luam tawm.

Peroxidase kuj tseem siv rau kev txiav txim siab ntawm qabzib 4 thiab peroxides hauv kev daws.Ntau cov ntawv tshaj tawm, 6-24 cov ntawv no, 25-29 thiab cov ntawv tshaj tawm 30-46 tau hais txog kev siv P8375 hauv lawv cov txheej txheem tshawb fawb.