Glycohemoglobin A1c (HbA1c) Test Kit
Qhov zoo
● Cov neeg raug siab heev
● Muaj peev xwm tiv thaiv kev cuam tshuam
● Kev ruaj ntseg zoo
Chemical Structure
Daim ntawv thov
Hauv vitro kuaj rau qhov kev txiav txim siab ntau ntawm HbA1c concentration hauv tib neeg cov ntshav ntawm cov tshuab photometric.HbA1c yog ib yam khoom ntawm hemoglobin (Hb) uas ua rau qeeb thiab tsis tu ncua tsis-enzymatic glycation cov tshuaj tiv thaiv nyob rau hauv cov ntshav siab.Glucose hloov kho hemoglobin tshwj xeeb hauv nws cov n-terminal valine residue los tsim glycated hemoglobin.Raws li ib txwm muaj lub cev nqaij daim tawv, kev tsim cov khoom tsis-enzymatic glycosylation cov tshuaj tiv thaiv yog qhov zoo rau cov concentration ntawm reactants.Txij li thaum hemoglobin concentration tseem nyob ruaj khov, qib glycosylation feem ntau yog nyob ntawm cov piam thaj concentration thiab tseem cuam tshuam txog qhov ntev ntawm hemoglobin thiab qabzib raug.Yog li, HbA1c yog qhov qhia tau zoo rau cov ntshav qabzib nruab nrab ntawm cov neeg mob rau 2 ~ 3 lub hlis dhau los.
Txoj Cai
Nyob rau hauv qhov kev txiav txim ntawm protease, lub n-terminal ntawm β saw hauv HbA1c raug txiav tawm thiab glycosylated dipeptides raug tso tawm.Hauv thawj cov tshuaj tiv thaiv, Hb concentration tuaj yeem tau los ntawm kev ntsuas qhov nqus ntawm 480 nm.Hauv qhov thib ob cov tshuaj tiv thaiv, fructosyl peptide oxidase (FPOX) ua rau glycosylated dipeptides los tsim hydrogen peroxide uas tuaj yeem hnov mob nrog cov kab mob chromogenic los ua kom muaj qhov nqus ntawm 660nm nyob rau hauv lub xub ntiag ntawm peroxidase, ces qhov concentration ntawm HbA1c tuaj yeem tau los ntawm kev ntsuas qhov nqus ntawm 660nm ua.Raws li qhov tau txais HbA1c concentration thiab Hb concentration, qhov feem pua ntawm HbA1c (HbA1c%) tuaj yeem suav.
Siv tau
Hitachi 7180/7170/7060/7600 tsis siv neeg biochemical tsom xam, Abbot 16000, OLYMPUS AU640 tsis siv neeg biochemical analyzer
Reagents
| Cheebtsam | Kev xav |
| Reagent 1 (R1) | |
| Good's buffer | 100 mmol / L |
| PRK | 500 KUB/L |
| UA-67 | 10 mmol / L |
| Reagents 2 (R2) | |
| Good's tsis | 100 mmol / L |
| Fructosyl peptide oxidase | 50 KUB/L |
| Reagent 3 (R3) | |
| Good's tsis | 100 mmol / L |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Ambient
Cia thiab Stability:
Txog hnub tas sij hawm teev tseg rau ntawm daim ntawv lo, thaum khaws cia tsis tau qhib ntawm 2-8 ℃ thiab tiv thaiv los ntawm lub teeb.Thaum qhib, cov reagents ruaj khov rau 28 hnub thaum tso tub yees rau ntawm lub ntsuas ntsuas lossis lub tub yees.
Kev sib kis ntawm cov reagent yuav tsum zam.Tsis txhob khov cov reagent.
Thaum yaj, lub calibrator ruaj khov rau 15 hnub ntawm 2-8 ℃, kev tswj tau ruaj khov rau 7 hnub ntawm 2-8 ℃, tsis txhob khov.
Lub neej txee:1 xyoo
