RT-LAMP Colormetric (Lyophilized pob)

Cov khoom no muaj cov tshuaj tiv thaiv tsis zoo, RT-Enzymes Mix (Bst DNA polymerase thiab kub-resistant reverse transcriptase), lyophilized tiv thaiv thiab chromogenic dye Cheebtsam.Cov khoom yog lyophilized pob hom, tsuas yog siv nrog primers thiab templates.Cov khoom siv no muab ceev, pom tseeb pom pom ntawm kev nthuav dav, uas cov tshuaj tiv thaiv tsis zoo yog qhia hauv liab thiab cov tshuaj tiv thaiv zoo yog qhia los ntawm kev hloov mus rau daj.

Cheebtsam

RT-LAMP Colormetric Master Mix (Lyophilized hlaws)

Daim ntawv thov

Rau DNA lossis RNA isothermal amplification.

Kev cia khoom

Thauj thiab khaws cia ntawm 2 ~ 8 ℃.Cov khoom siv tau rau 12 lub hlis.

raws tu qauv

1.Tshem tawm tus lej sib raug Lyophilized hlaws hmoov raws li tus naj npawb ntawm kev xeem.

2.Npaj cov tshuaj tiv thaiv sib tov

Nco tseg:

1. 10 × Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;

2. Nucleic acid templates raug pom zoo kom yaj siv DEPC dej.

3.Incubate ntawm 65 ° C rau 30-45mins, uas tuaj yeem txuas ntxiv kom tsim nyog raws li xim hloov Lub Sijhawm Tawm.

4.Raws li qhov muag liab qab, daj yog qhov zoo thiab liab tsis zoo.

Sau ntawv

1.Cov tshuaj tiv thaiv kub tuaj yeem ua kom zoo ntawm 62 ℃ thiab 68 ℃ raws li cov txheej txheem primer.

2.Cov pob reagents yuav tsum tsis txhob raug cua rau lub sijhawm ntev.

3.Cov tshuaj tiv thaiv xim liab thiab daj yog nyob ntawm pH hloov ntawm cov tshuaj tiv thaiv kab mob, thov tsis txhob siv cov tshuaj Tris nucleic acid cia, pom zoo kom siv ddH₂O khaws cia nucleic acid.

4.Qhov kev sim yuav tsum tau ua raws li tus qauv, nrog rau kev npaj cov tshuaj tiv thaiv kab mob, kev kho cov qauv thiab cov qauv ntxiv.

5.Nws raug nquahu kom npaj cov tshuaj tiv thaiv kab mob hauv lub rooj ua kom huv huv thiab ntxiv cov qauv hauv cov fume hood ntawm lwm chav kom tsis txhob muaj qhov cuav.