RT-LAMP Fluorencent Master Mix

Cat No: HCB5205A

Pob: 96RXN / 960RXN / 9600RXN

Cov khoom no muaj cov tshuaj tiv thaiv tsis zoo, RT-Enzymes Mix (Bst DNA polymerase thiab kub-resistant reverse transcriptase), lyophilized Protectants thiab chromogenic dye Cheebtsam.

Xa lus nug

Product Description

Khoom nthuav dav

Cov khoom no muaj cov tshuaj tiv thaiv tsis zoo, RT-Enzymes Mix (Bst DNA polymerase thiab kub-resistant reverse transcriptase), lyophilized Protectants thiabfluorescentdye Cheebtsam.Cov tshuaj tiv thaiv tsis muaj Mg2+, dNTP thiab lwm yam khoom tsim nyog rau kev nthuav dav.Txhawm rau siv, tsuas yog sib xyaw Buffer, cov tshuaj tiv thaiv enzyme, fluorescent dye, primer thiab ntxiv cov qauv, ntxiv cov tshuaj tiv thaiv lyophilized tuaj yeem siv ncaj qha.Nws tau txuas nrog lyophilizer thiab lyophilized, thiab tsuas yog cov primers thiab templates tau ntxiv thaum siv.Cov reagent no muaj kev ua haujlwm siab amplification thiab rhiab heev.

Yav dhau los: RT-LAMP Fluorescent (Lyophilized pob) HCB5207A

Tom ntej: Bst 2.0 DNA Polymerase (Glycerol dawb, siab ceev) HC5007A

Cheebtsam

Cheebtsam	HCB5205A-01	HCB5205A-02	HCB5205A-03
Loop-mediated Amplification Buffer	1.2 ml ib	4ml x 3	12 mL x 10
RT-Enzymes Mix	245l ua	2.45 ml ib	2.45 hli × 10
Lyophilized tiv thaiv	0.96ml x 2	9.60ml x 2	9.60ml x 20
25 × Dye	192l ua	0.96ml x 2	0.96 hli × 20

Daim ntawv thov

Rau DNA lossis RNA isothermal amplification.

Kev cia khoom

Thauj nrog dej khov qhuav, khaws cia ntawm -25 ~ -15 ℃.Tsis txhob nquag khov-thaw, cov khoom siv tau rau 12 lub hlis.

raws tu qauv

1.Thaw cov tshuaj tiv thaiv tsis tau siv rau hauv chav tsev kub.Vortex luv luv los yog invert hlab ob peb zaug kom sib tov kom huv si, ces centrifuge los sau cov kua rau hauv qab ntawm lub raj.

2.Kev npaj cov tshuaj tiv thaiv kab mob.Cov reagent no tuaj yeem npaj tau hauv ob lub tshuab tshuaj tiv thaiv, kua tshuaj tiv thaiv sib xyaw thiab lyophilized system mix.

1) Npaj cov tshuaj tiv thaiv ua kua

Cheebtsam	Ntim
Loop-mediated Amplification Buffer	12.5 hli
25 × Dye	2l miv
RT-Enzymes Mix	2,55l ua
10 × Primer Mix^a	5l miv
Templates DNA/RNA^b	× μL
Dej tsis muaj Nuclease	Txog li 50 μL

2) Lyophilization system sib xyaw

① Npaj lyophilized mix

Cheebtsam	Ntim
Loop-mediated Amplification Buffer	12.5 hli
Lyophilized tiv thaiv	20 mL
25 × Dye	2l miv
RT-Enzymes Mix	2,55l ua
Dej tsis muaj Nuclease	Txog li 50 μL

② Lyophilization: Cov kev sib xyaw npaj tau lyophilized hauv 50μL system

③ Npaj cov tshuaj tiv thaiv

Cheebtsam	Ntim
Lyophilized sib tov	1 qob
10 × Primer Mix^a	5l miv
Templates DNA/RNA^b	× μL
Dej tsis muaj Nuclease	Txog li 50 μL

Nco tseg:

1) a.10 × Primer Mix: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;

2) ib.DEPC (dej soluble) yog pom zoo rau nucleic acid templ.

3)Amplification Reaction: Teeb qhov kev pab cuam hauv qab no ntawm lub ntsuas hluav taws xob fluorescent PCR (xws li ABI7500, thiab lwm yam):

Kub	Sijhawm	Vajvoog
65 ℃	60 sec * Sau FAM fluorescence	30

Sau ntawv

1.Cov ntsev tuaj yeem tshwm sim hauv qab ntawm lub raj tsis muaj, vortex luv luv los yog cov raj invert ob peb zaug kom sib tov kom huv hauv chav sov.

2.Cov tshuaj tiv thaiv kub tuaj yeem ua kom zoo ntawm 62 ℃ thiab 68 ℃ raws li cov txheej txheem ntawm primers.

3.Qhov kev sim yuav tsum yog tus qauv, suav nrog kev npaj cov tshuaj tiv thaiv kab mob, lyophilization, thiab cov qauv ua qauv thiab cov qauv ntxiv cov txheej txheem;

4.Txhawm rau kom tsis txhob muaj kev sib kis, nws raug nquahu kom npaj cov tshuaj tiv thaiv nyob rau hauv lub rooj ntev zaum ultra-huv, hauv lwm cov qauv ntxiv rau lub fume hood ntawm chav tsev kom tsis txhob muaj kev cuam tshuam tsis zoo.