RT-LAMP Colormetric Master Mix HCB5204A
Cov khoom no muaj cov tshuaj tiv thaiv tsis zoo, RT-Enzymes Mix (Bst DNA polymerase thiab kub-resistant reverse transcriptase), lyophilized Protectants thiab chromogenic dye Cheebtsam.Txhawm rau siv, tsuas yog siv Buffer, cov tshuaj tiv thaiv enzyme thiab primer yog tov thiab ntxiv rau cov qauv;ntxiv lyophilized tiv thaiv tuaj yeem ncaj.Nws tau txuas nrog lyophilizer thiab lyophilized, thiab tsuas yog cov primers thiab templates tau ntxiv thaum siv.Cov khoom siv no muab ceev, pom tseeb pom pom ntawm kev nthuav dav, uas cov tshuaj tiv thaiv tsis zoo yog qhia hauv liab thiab cov tshuaj tiv thaiv zoo yog qhia los ntawm kev hloov mus rau daj.
Cheebtsam
| Cheebtsam | HCB5204A-01 | HCB5204A-02 | HCB5204A-03 |
| Loop-mediated Amplification Buffer (nrog dye) | 0.96ml ib | 4.80ml x 2 | 9.60 mL x 10 |
| RT-Enzymes Mix | 270l ua | 2.70ml ib | 2.70 hli × 10 |
| Lyophilized tiv thaiv | 0.96ml x 2 | 9.60ml x 2 | 9.60ml x 20 |
Daim ntawv thov
Rau DNA lossis RNA isothermal amplification.
Kev cia khoom
Thauj nrog dej khov qhuav, khaws cia ntawm -25 ~ -15 ℃.Tsis txhob nquag khov-thaw, cov khoom siv tau rau 12 lub hlis.
raws tu qauv
1.Thaw cov tshuaj tiv thaiv tsis tau siv rau hauv chav tsev kub.Vortex luv luv los yog invert hlab ob peb zaug kom sib tov kom huv si, ces centrifuge los sau cov kua rau hauv qab ntawm lub raj.
2.Kev npaj cov tshuaj tiv thaiv kab mob.Cov reagent no tuaj yeem npaj tau hauv ob lub tshuab tshuaj tiv thaiv, kua tshuaj tiv thaiv sib xyaw thiab lyophilized system mix.
1) Npaj cov tshuaj tiv thaiv ua kua
| Cheebtsam | Ntim |
| Loop-mediated Amplification Buffer (nrog dye) | 10 mL |
| RT-Enzymes Mix | 2,8l ua |
| 10 × Primer Mixa | 5l miv |
| Templates DNA/RNA b | × μL |
| Dej tsis muaj Nuclease | Txog li 50 μL |
2) Lyophilization system sib xyaw
① Npaj lyophilized mix
| Cheebtsam | Ntim |
| Loop-mediated Amplification Buffer (nrog dye) | 10 mL |
| Lyophilized tiv thaiv | 20 mL |
| RT-Enzymes Mix | 2,8l ua |
| Dej tsis muaj Nuclease | Txog li 50 μL |
② Lyophilization: Cov kev sib xyaw npaj tau lyophilized hauv 50μL system
③ Npaj cov tshuaj tiv thaiv
| Cheebtsam | Ntim |
| Lyophilized sib tov | 1 qob |
| 10 × Primer Mixa | 5l miv |
| Templates DNA/RNA b | × μL |
| Dej tsis muaj Nuclease | Txog li 50 μL |
Nco tseg:
1) a.10 × Primer Mix: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) ib.DEPC (dej soluble) yog pom zoo rau nucleic acid templ.
1.Incubate ntawm 65 ° C rau 30-45mins, uas tuaj yeem txuas ntxiv kom tsim nyog raws li xim hloov Lub Sijhawm Tawm.
2.Raws li qhov muag liab qab, daj yog qhov zoo thiab liab tsis zoo.
Sau ntawv
1.Cov ntsev tuaj yeem tshwm sim hauv qab ntawm lub raj tsis muaj, vortex luv luv los yog cov raj invert ob peb zaug kom sib tov kom huv hauv chav sov.
2.Cov tshuaj tiv thaiv kub tuaj yeem ua kom zoo ntawm 62 ℃ thiab 68 ℃ raws li cov txheej txheem ntawm primers.
3.Cov pob reagents yuav tsum tsis txhob raug cua rau lub sijhawm ntev.
4.Cov tshuaj tiv thaiv xim liab thiab daj yog nyob ntawm pH hloov ntawm cov tshuaj tiv thaiv kab mob, thov tsis txhob siv cov tshuaj Tris nucleic acid cia, pom zoo kom siv ddH2O khaws cia nucleic acid;
5.Qhov kev sim yuav tsum yog tus qauv, suav nrog kev npaj cov tshuaj tiv thaiv kab mob, lyophilization, thiab cov qauv ua qauv thiab cov qauv ntxiv cov txheej txheem;
6.Txhawm rau kom tsis txhob muaj kev sib kis, nws raug nquahu kom npaj cov tshuaj tiv thaiv nyob rau hauv lub rooj ntev zaum ultra-huv, hauv lwm cov qauv ntxiv rau lub fume hood ntawm chav tsev kom tsis txhob muaj kev cuam tshuam tsis zoo.
