T4 RNA ligase
Kev piav qhia
Cov khoom no yog ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly qhia los ntawm E. coli.Qhov no enzyme catalyzes tsim cov phosphodiester bonds ntawm oligonucleotides, ib leeg-stranded RNA thiab DNA intermolecular / intra-molecular 5'-PO4 thiab 3'-OH.
Unit txhais
Ib chav tsev yog txhais raws li tus nqi ntawm cov enzyme uas yuav tsum tau hloov 1 nanomole ntawm 5´-[³²P] rA16 rau hauv daim ntawv phosphatase-resistant hauv 30 feeb ntawm 37 ° C.
Specification
| Cov khoom kuaj | Specifications |
| Kev ua enzyme | 10 U / μL |
| Protein Activity | ≥ 95% |
| Nickase | Tsis ntes tau |
| RNase Kev Ua Haujlwm | Tsis ntes tau |
| Ecoli gDNA | ≤1 daim / 50U |
| Endotoxin | LCL-Test, ≤10EU / mg |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Dej khov qhuav
Cia:Khaws ntawm -25 ~ -15 ° C (tsis txhob rov khov thiab thawing)
Pom zoo rov xeem lub neej:2 xyoo
cov khoom muaj feem xyuam
Sau koj cov lus ntawm no thiab xa tuaj rau peb
