Homocysteine (HCY)
Kev piav qhia
Homocysteine (HCY) yog siv los kuaj homocysteine hauv tib neeg cov ntshav.Homocysteine (Hcy) yog sulfur-muaj amino acid tsim los ntawm cov metabolism ntawm methionine.80% ntawm Hcy yog khi rau cov proteins los ntawm disulfide bonds nyob rau hauv cov ntshav, thiab tsuas yog ib feem me me ntawm dawb homocysteine koom nyob rau hauv txoj kev.Hcy qib muaj feem cuam tshuam rau cov kab mob plawv.yog ib qho tseem ceeb ntawm kev pheej hmoo rau kab mob plawv.Qhov nce Hcy hauv cov ntshav txhawb nqa cov hlab ntsha phab ntsa ua rau muaj kev puas tsuaj rau cov hlab ntsha, ua rau o thiab cov plaque tsim ntawm phab ntsa ntawm cov hlab ntsha, uas nws thiaj li ua rau cov ntshav txhaws hauv lub plawv.Hauv cov neeg mob uas muaj hyperhomocystinuria, cov kab mob loj heev cuam tshuam rau Hcy metabolism, ua rau hyperhomocysteineemia.Cov kab mob me me lossis kev noj zaub mov tsis zoo ntawm cov vitamins B yuav ua nrog kev nce qib ntawm Hcy nruab nrab lossis me me, uas yuav ua rau muaj kev pheej hmoo ntawm kab mob plawv.Elevated Hcy tuaj yeem ua rau muaj qhov tsis xws luag thaum yug los xws li neural tube defects thiab congenital malformations.
Chemical Structure
Test txoj cai
Oxidized Hcy hloov mus rau Hcy dawb, thiab dawb Hcy reacts nrog serine nyob rau hauv catalysis ntawm CBS los tsim L-cystathionine.L-cystathionine tsim Hcy, pyruvate thiab NH3 nyob rau hauv catalysis ntawm CBL.Cov pyruvate tsim los ntawm lub voj voog cov tshuaj tiv thaiv no tuaj yeem kuaj pom los ntawm lactate dehydrogenase LDH thiab NADH, thiab qhov hloov pauv ntawm NADH rau NAD yog ncaj qha proportional rau Hcy cov ntsiab lus hauv cov qauv.
Kev thauj mus los thiab khaws cia
Kev thauj mus los:2-8 ° C
Lub sijhawm cia thiab siv tau:Unopened reagents yuav tsum khaws cia ntawm 2-8 ° C hauv qhov tsaus ntuj, thiab lub sijhawm siv tau yog 12 lub hlis;Tom qab qhib, cov reagents yuav tsum tau muab cia rau hauv qhov tsaus ntuj ntawm 2-8 ° C, thiab lub sijhawm siv tau yog 1 lub hlis nyob rau hauv qhov xwm txheej tsis muaj kuab paug;cov reagents yuav tsum tsis txhob khov.
Nco tseg
Cov qauv xav tau: Cov qauv yog cov ntshav ntshav tshiab lossis ntshav ntshav (heparin anticoagulation, 0.1mg heparin tuaj yeem tiv thaiv ntshav 1.0ml).Thov centrifuge lub plasma tam sim ntawd tom qab sau cov ntshav, los yog refrigerate thiab centrifuge nyob rau hauv 1 teev.
