Taqman Multiplex qPCR Premix
Cat No: HCB5061B
Taqman Multiplex qPCR Premix master mix yog ib tiam tshiab antibody hloov Taq DNA Polymerase fluroescence quantitative qPCR tuaj yeem siv rau hauv lyophilization reagent.Nws yog tsim rau FAM / HEX / TET / JOE / ROX thiab lwm yam. Ob-labeled probes (tseem haum rau Molecular Beacon probes).Cov reagent muaj hotstart Fast Taq DNA Polymerase, dNTP thiab lwm yam khoom uas tuaj yeem ua dawb-qhuav ncaj qha rau ntawm lub tshuab.Cov khoom tsis muaj ROX-kho dyes thiab yog haum rau ntau yam PCR qauv.
Cheebtsam
Lyo-Ready TaqMan qPCR Mix
Kev cia khoom
Cov khoom no yuav tsum khaws cia ntawm -25 ~ -15 ℃ deb ntawm lub teeb rau 1 xyoos.
Cov lus qhia
1.Cov tshuaj tiv thaiv kab mob
| Cheebtsam | Ntim (μL) | Thaum kawg concentration |
| Enzyme Mix | 20 | - |
| Primer (10μM) | x | 0.1-0.5μM |
| Kev sojntsuam Primer (10μM) | x | 50-250 nM |
| DNA Tamplate | 1-25 | - |
| dd H2O | mus 25 | - |
2.Reaction program
| Lub voj voog kauj ruam | Temp. | Sijhawm | Lub voj voog |
| Thawj denaturation | 95 ℃ | 5 feeb | 1 |
| Amplification cov tshuaj tiv thaiv | 95 ℃ | 15 sec | 45 |
| Annealing / Extension | 60 ℃ | 30 sec |
Nco tseg:Hauv cov xwm txheej tshwj xeeb, qhov kub thiab txias tuaj yeem hloov kho raws li tus nqi primer Tm, 60 ° C raug pom zoo.
Txheej txheem Lyophilization
| Txheej txheem | Temp. | Teem sijhawm | Lub sijhawm ntev | Siab |
| Ua ntej khov | -45 ℃ | 10 min | 60 feeb | — |
|
Kev ziab qhuav | -45 ℃ | 10 min | 60 feeb | 0,01m wb |
| -40 ℃ | 10 min | 600 min | 0,01m wb | |
| -35 ℃ | 10 min | 120 min | 0,01m wb | |
| -30 ℃ | 20 feeb | 60 feeb | 0,01m wb | |
| -20 ℃ | 20 feeb | 60 feeb | 0,01m wb | |
| -10 ℃ | 20 feeb | 60 feeb | 0,01m wb | |
| 0 ℃ | 20 feeb | 60 feeb | 0,01m wb | |
|
Secondary Drying | 5 ℃ | 10 min | 20 feeb | 0,01m wb 0,01m wb |
| 10 ℃ | 10 min | 20 feeb | ||
| 15 ℃ | 10 min | 20 feeb | 0,01m wb | |
| 20 ℃ | 10 min | 20 feeb | 0,01m wb | |
| 25 ℃ | 10 min | 240 min | 0,01m wb |
Sau ntawv
1. Cov khoom no yog siv rau kev tshawb fawb nkaus xwb.
2. Thov ua haujlwm nrog cov tsho hauv tsev kuaj mob thiab cov hnab looj tes pov tseg, kom koj muaj kev nyab xeeb.
