Ultra Nuclease
UltraNuclease yog ib qho genetic engineeredendonuclaese muab tau los ntawm Serratia marcescens, uas muaj peev xwm degrade DNA los yog RNA, ob leeg los yog ib leeg stranded, linear los yog ncig nyob rau hauv ib tug ntau yam ntawm cov mob, ua tiav degrade nucleic acids rau hauv 5'-monophosphate oligonucleotides ntev. .Tom qab kev hloov kho cov noob caj noob ces, cov khoom lag luam tau fermented, qhia, thiab purifiedin Escherichia coli (E. coli), uas txo cov viscosity ntawm cell supernatant thiab cell lysate inscientific kev tshawb fawb, tab sis kuj txhim kho cov purification efficiency thiab functional research ofprotein.Nws kuj tuaj yeem siv rau hauv kev kho noob, kho tus kab mob, kev tsim tshuaj tiv thaiv, kev lag luam tshuaj protein thiab polysaccharide ua tus tswv tsev residue nucleic acid tshem tawm reagent.
Khoom nta
| CAS Nr. | 9025-65-4 : kuv |
| EC No. | |
| Molecular Luj | 30kd ua |
| Isoelectric Point | 6.85 ib |
| Protein Purity | ≥99% (SDS-PAGE & SEC-HPLC) |
| Kev ua tshwj xeeb | ≥1.1 × 106 U / mg |
| OptimumTemperature | 37 ° C |
| Qhov zoo tshaj pH | 8.0 ib |
| ProteaseActivity | tsis zoo |
| Bioburden | 10 CFU / 100,000U |
| Residual Host-cell Protein | ≤10ppm |
| Hnyav Hlau | ≤10ppm |
| Cov kab mob endotoxin | 0.25EU/1000U |
| Storage Buffer | 20mM Tris-HCl, pH 8.0, 2mM MgCl2, 20mM NaCl, 50% Glycerol |
Cia tej yam kev mob
≤0 ° C kev thauj mus los; -25 ~ -15 ° C Cia, 2 xyoos siv tau (tsis txhob khov-thawing).
Unit txhais
Tus nqi ntawm cov enzyme siv los hloov tus nqi nqus ntawm △A260 los ntawm 1.0 nyob rau hauv 30min ntawm 37 ° C, pH 8.0, sib npaug rau digested 37μg salmon phev DNA los ntawm kev txiav rau hauv oligonucleotides, tau txhais tias yog ib qho active unit (U).
Kev tswj kom zoo
Residual Host-cell Protein: ELISA kit
•Protease Cov seem: 250KU/mL UltraNuclease reacted nrog substrate rau 60min, tsis muaj kev ua si.
•Cov kab mob endotoxin: LAL-Test, Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition) gel limit test method.Cov cai dav dav (1143).
•Bioburden: Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition)—Txhua
Cov cai rau Sterility Test (1101), PRC National Standard, GB 4789.2-2016.
•Heavy Hlau:ICP-AES, HJ776-2015.
Kev ua haujlwm
UltraNuclease kev ua haujlwm tau cuam tshuam loj heev thaum SDS concentration ntau dua 0.1% lossis EDTA
Qhov siab tshaj 1mM.Surfactant Triton X-100, Tween 20 thiab Tween 80 tsis muaj kev cuam tshuam rau nuclease.
thaum lub concentration yog qis dua 1.5%.
| Kev ua haujlwm | Kev ua haujlwm zoo | Kev ua haujlwm raug |
| Kub | 37 ℃ | 0-45 ℃ |
| pH | 8.0-9.2 Nws | 6.0-11.0 Nws |
| Mg2 + | 1-2 mM | 1-15 hli |
| DTT | 0-100 hli | > 100 hli |
| 2-Mercaptoethanol | 0-100 hli | > 100 hli |
| Monovalent hlau ion (Na+, K+ etc.) | 0-20 hli | 0-200 hli |
| PO 43- | 0-10 hli | 0-100 hli |
Kev siv thiab siv
• Tshem tawm cov exogenous nucleic acid los ntawm cov khoom siv tshuaj tiv thaiv, txo qhov kev pheej hmoo ntawm residual nucleic acid toxicity thiab txhim kho cov khoom ruaj ntseg.
• Txo cov viscosity ntawm cov kua pub los ntawm nucleic acid, txo lub sij hawm ua thiab ua kom cov protein ntau.
• Tshem tawm cov nucleic acid uas qhwv particle (virus, inclusion body, etc.), uas yog conducive.
mus rau kev tso tawm thiab purification ntawm particle.
| Hom kev sim | Protein Production | Kab mob, tshuaj tiv thaiv | Tshuaj Cell |
| Cells Number | 1 g cell ntub dej hnyav (resuspended nrog 10ml buffer) | 1L yog fermentation kua supernatant | 1 l culture |
| Yam tsawg kawg nkaus | 250 ua | 100 ua | 100 ua |
| Pom zoo noj | 2500 ua | 25 000 U | 5000 ua |
• Kev kho mob Nuclease tuaj yeem txhim kho qhov kev daws teeb meem thiab rov qab los ntawm cov qauv rau kab chromatography, electrophoresis thiab kev tsom xam blotting.
• Hauv kev kho noob caj noob ces, nucleic acid raug tshem tawm kom tau txais cov kab mob purified adeno-associated.
