BsaI txwv Endonuclease

Purity: 20U / μL

Pob: 50μL, 250μL, 1ml, 10ml, 100ml

Xa lus nug

Product Detail

Kev piav qhia

BsaI txwv enzyme yog hom II txwv endonuclease tau los ntawm cov protein recombinant encoded los ntawm BspQI noob hauv Bacillus sphaericus qhia los ntawm E.coli.Kev txwv endonucleases tau siv dav hauv kev cog qoob loo, cloning thiab lwm yam.Qhov enzyme no sai sai txiav DNA rau cov noob tau zoo linearization Bsa I, ib qho IIs txwv endonuclease txwv endonuclease, yog muab los ntawm ib tug recombinant E. coli hom uas nqa cov cloned thiab hloov BsaI noob los ntawm Bacillus stearothermophilus.uas muaj tib yam tshwj xeeb raws li haiv neeg enzymes thiab txo cov hnub qub ua haujlwm.Nws qhov kev lees paub ua ntu zus thiab qhov chaw cleavage yog raws li hauv qab no:

5'·······GTCTC(N)············

Chemical Structure

Khoom nta

• Cov kev txwv endonuclease muaj kev ua haujlwm siab enzymatic thiab ua tiav kev zom zaub mov sai.

• Tsis tshua muaj kev ua haujlwm endonuclease kom paub meej "scalpel" txiav.

• Tsis muaj BSA system, txo cov cua sov qhov chaw.

Specification

Cov khoom kuaj	Specifications
Kev ua enzyme	20 U/µL
Purity (SDS-PAGE)	≥95%
Rnase	Tsis pom muaj leej twg
Tsis-Specific DNase Activity	Tsis kuaj pom
Hnub Qub Kev Ua Si	Tsis kuaj pom
Ligation thiab Recutting	Tom qab kev zom zaub mov, DNA tawg tuaj yeem raug ligated.Thiab cov tawg tawg no tuaj yeem raug txiav nrog Bsal.
Endotoxins (LAL-test)	≤10 EU / mg
E.Coli DNA	≤1 Daim / 2U

Kev tswj kom zoo

Purity ≥ 95%, Tsis muaj DNase, RNase kev ua, Tus Tswv DNA residue ≤ 100pg / mg, host protein residue ≤ -50ppm, endotoxin residue ≤ 10EU / mg, Tsis muaj kev ua haujlwm protease, tsis muaj menyuam, tsis muaj mycoplasma

Daim ntawv thov

Digest lub plasmid los npaj ib tug linearized DNA fragment kawg ntawm Poly (A/T/C/G);

Linearize plasmid template ua ntej hauv vitro transcription;

Kev txwv digest;

Unit txhais

Ib chav tsev yog txhais raws li tus nqi ntawm cov enzyme uas yuav tsum tau zom 1 µg ntawm Internal tswj DNA hauv 1 teev ntawm 37 ° C hauv tag nrho cov tshuaj tiv thaiv ntim ntawm 50 µL