Ultra Nuclease Assay Kit (ELISA)

CAS Nr .: 9025-65-4

EC.Nr.: 3.1.30.2

Pob: 96 Test

Xa lus nug

Product Detail

Kev piav qhia

Cov khoom siv Ultra Nuclease ELISA no yog cov xuab zeb uas ELISA yuav tsum tau ua nyob rau hauv ib hom microplate.Cov qauv uas muaj cov endonuclease yog incubated nyob rau hauv microtiter phaj qhov dej uas tau pre-coated nrog affinity purified anti-endonuclease ntes antibody.Tom qab incubation thiab ntxuav cov kauj ruam nyob rau hauv uas unbound Cheebtsam raug tshem tawm, ib tug enzyme-conjugated, anti-endonuclease detector antibody ntxiv.Cov tshuaj tiv thaiv kab mob hauv lub cev ua rau muaj kev tsim cov txheej txheem nyuaj ntawm cov theem khoom antibody-endonuclease-enzyme sau npe antibody.Tom qab qhov kawg ntxuav cov kauj ruam, cov tshuaj substrate yog ntxiv rau hauv qhov dej thiab reacted, ua rau cov xim tsim.Lub optical ceev yog ntsuas photometrically thiab yog proportional rau lub
tsom xam concentration tam sim no nyob rau hauv lub qhov dej.Lub endonuclease concentration hauv cov qauv tsis paub yuav raug xam raws li tus qauv nkhaus.

Chemical Structure

Unit txhais

Tus nqi ntawm enzyme siv los hloov tus nqi nqus ntawm △A260 los ntawm 1.0 hauv 30min
ntawm 37 ° C, pH 8.0, sib npaug rau digested 37μg salmon phev DNA los ntawm kev txiav rau hauv oligonucleotides, tau txhais tias yog ib qho chaw ua haujlwm.

Kev siv thiab siv

• Tshem tawm cov exogenous nucleic acid los ntawm cov khoom siv tshuaj tiv thaiv, txo qhov kev pheej hmoo ntawm residual nucleic acid toxicity thiab txhim kho cov khoom ruaj ntseg.
• Txo cov viscosity ntawm cov kua pub los ntawm nucleic acid, txo lub sij hawm ua thiab ua kom cov protein ntau.
• Tshem tawm cov nucleic acid uas qhwv cov kab mob (tus kab mob, suav nrog lub cev, thiab lwm yam), uas yog qhov tsim nyog rau kev tso tawm thiab ua kom huv ntawm cov khoom.
• Kev kho mob Nuclease tuaj yeem txhim kho qhov kev daws teeb meem thiab rov qab los ntawm cov qauv rau kab chromatography, electrophoresis thiab kev tsom xam blotting.
• Hauv kev kho noob caj noob ces, nucleic acid raug tshem tawm kom tau txais cov kab mob purified adeno-associated.