Alkaline Phosphatase (ALP)

Alkaline Phosphatase yog muab los ntawm ib tug recombinant E. coli hom uas nqa cov noob TAB5.Lub enzyme catalyzes dephosphorylation ntawm 5' thiab 3' kawg ntawm DNA thiab RNA phosphomonoesters.Tsis tas li ntawd, nws hydrolyses ribose, nrog rau deoxyribonucleoside triphosphates (NTPs thiab dNTPs).TAB5 Alkaline Phosphatase ua rau 5' protruding, 5' recessed thiab blunt kawg.Phosphatase tuaj yeem siv rau hauv ntau daim ntawv thov kev lom neeg molecular, xws li cloning lossis sojntsuam kawg daim ntawv lo kom tshem tawm cov phosphorylated kawg ntawm DNA lossis RNA.Hauv kev sim cloning, dephosphorylation tiv thaiv cov linearized plasmid DNA los ntawm tus kheej-ligation.Nws tseem tuaj yeem degrade unincorporated dNTPs hauv PCR cov tshuaj tiv thaiv los npaj cov qauv rau DNA sequencing.Lub enzyme yog tag nrho thiab irreversibly inactivated los ntawm cua sov ntawm 70 ° C rau 5 feeb, yog li ua kom tshem tawm cov phosphatase ua ntej ligation los yog xaus daim ntawv lo tsis tsim nyog.