D-Lactate dehydrogenase (LDH)
Kev piav qhia
Cov enzyme yog siv rau kev tshem tawm ntawm pyruvate hauv kev txiav txim siab ua haujlwm nrog NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Kev siv
1.Alkaline phosphatase ua ke rau cov proteins (antibodies, streptavidin thiab lwm yam,) tuaj yeem txheeb xyuas cov hom phiaj molecules, thiab tuaj yeem siv rau hauv ELISA, WB thiab kuaj histochemical;
2.Alkaline phosphatase tuaj yeem siv los dephosphorize 5 '-terminal ntawm DNA lossis RNA los tiv thaiv kev sib txuas ntawm tus kheej;
3.Qhov saum toj no dephosphorylated DNA lossis RNA tuaj yeem sau los ntawm xov tooj cua-labeled phosphates (ntawm T4 poly-nucleotide kinase)
Chemical Structure
Cov tshuaj tiv thaiv mechanism
D-Lactate + NAD+→ Pyruvate + NADH + H+
Specification
| Cov khoom kuaj | Specifications |
| Kev piav qhia | Dawb amorphous hmoov, lyophilized |
| Kev ua si | ≥ 200 U / mg |
| Purity (SDS-PAGE) | ≥90% |
| Solubility (10mg hmoov / ml) | Ntshiab |
| NADH / NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤ 0.005% |
| Glutamate dehydrogenase | ≤ 0.003% |
| Pyruvate kinase | ≤ 0.03% |
| Glutamate Dehydrogenase | ≤ 0.003% |
| Aspartate aminotransferase | ≤ 0.001% |
| Alamine aminotransferase | ≤ 0.001% |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:AmbIent
Cia:Khaws ntawm -20 ° C (Lub Sij Hawm Ntev), 2-8 ° C (lub sijhawm luv)
Pom zoo rov sim duaLub neej:2 xyoo
