Glucose Dehydrogenase (GDH)
Kev piav qhia
Glutamate Dehydrogenase (GDH) yog ib qho mitochondrial enzyme uas catalyzes cov reversible oxidative deamination ntawm glutamate rau a-ketoglutarate thiab ua hauj lwm raws li ib tug tseem ceeb txuas ntawm anabolic thiab catabolic txoj kev.Hauv cov tsiaj nyeg, GDH yog raws li kev cai allosteric thiab muaj kev ua haujlwm siab hauv siab, raum, hlwb, thiab txiav txiav.GDH kev ua hauv cov ntshav qab zib tuaj yeem siv los sib txawv ntawm cov kab mob siab vim lub siab mob, uas tsis qhia cov ntshav siab GDH kev ua, thiab cov kab mob uas ua rau hepatocyte necrosis, uas ua rau cov ntshav siab GDH.
GDH kev ua haujlwm yog txiav txim siab los ntawm kev sib xyaw ua ke enzyme uas siv glutamate los ntawm GDH tsim NADH, uas cuam tshuam nrog kev sojntsuam tsim cov khoom lag luam colorimetric (450 nm) piv rau GDH kev ua tam sim no.Ib chav tsev ntawm GDH yog cov enzyme uas yuav tsim 1.0 mmole ntawm NADH ib feeb ntawm pH 7.6 ntawm 37 ° C.
Chemical Structure
Cov tshuaj tiv thaiv mechanism
D-Glucose + txais → D-Glucono-1,5-lactone + txo qhov txais
Specification
| Cov khoom kuaj | Specifications |
| Kev piav qhia | Dawb amorphous hmoov, lyophilized |
| Kev ua si | ≥160U/mg |
| Purity (SDS-PAGE) | ≥90% |
| Solubility (10 mg hmoov / ml) | Ntshiab |
| Tiv thaiv cov enzymes | |
| Glucose dehydrogenase (NAD) | ≤ 0.02% |
| Hexokinase | ≤ 0.02% |
| A-Glucosidase | ≤ 0.02% |
Kev thauj mus los thiab khaws cia
Kev thauj mus los: Cov hnab ntim dej khov
Cia:Khaws ntawm -25 ~ -15 ° C (ntev ntev), 2-8 ° C (lub sijhawm luv)
Pom zoo rov sim duaLub neej: 18 hli
