Multiplex Ib Kauj Ruam RT-qPCR Premix
Kev piav qhia
Cat No: HCR5141A
Multiplex Ib Kauj Ruam RT-qPCR Premix yog Multiplex Quantitative PCR Cov Khoom Siv raws li RNA ua qauv.Hauv kev sim, thim rov qab thiab ntau PCR tau ua nyob rau hauv tib lub raj tshuaj tiv thaiv, ua kom yooj yim rau kev sim ua haujlwm thiab txo qhov kev pheej hmoo ntawm kev sib kis.Cov qauv tsim tshwj xeeb ntawm tsis muaj thiab enzyme sib xyaw tuaj yeem siv rau hauv ib-kauj ruam lyophilized system.Cov khoom siv siv hluav taws kub-resistant Reverse Transcriptase rau kev sib xyaw ua ke ntawm thawj strand cDNA siv hotstart Taq DNA Polymerase rau ntau qhov kev nthuav dav.Nws muaj cov tshuaj tiv thaiv zoo tshaj plaws, cov enzymes sib xyaw thiab lwm yam, thiab cov yam ntxwv uas cuam tshuam tsis zoo rau PCR amplification thiab txhim khu kev ua kom muaj zog ntawm ntau qhov qPCR cov tshuaj tiv thaiv tau ntxiv, ua kom muaj ntau yam fluorescence kom muaj nuj nqis amplification thaum ua kom cov amplification efficiency ntawm primers.
Cheebtsam
| Lub npe |
| 1. Lyo-Buffer |
| 2. Lyo-Enzyme Mix |
| 3. Lyo tiv thaiv |
Kev thauj mus losion
A: Lyo-buffer thiab tiv thaiv: -25 ~ -15 ℃, txee lub neej yog 1 xyoo.
B: Lyo-enzyme mix, 2-8 ℃, txee lub neej yog 6 lub hlis.
Kev qhia rau kev ua haujlwm
1. Cov tshuaj tiv thaiv kab mob (Siv 25μL ua piv txwv)
| Cheebtsam | Ntim (μL) | Thaum kawg Concentration |
| Lyo-Buffer | 6 | 1* |
| Lyo-Enzyme Mix | 1 | - |
| Lyo-Cov tshuaj tiv thaiv | 8 | - |
| Primer Mix (10μM) | 1 | 0.1-1.0 Nws |
| Kev sojntsuam Mix (10μM) | 0.5 | 0.05-0.5M 0.00 Nws |
| RNA Template | 5 | - |
| PEB H2O | Mus txog 25 | - |
2. Optimized Cycling Protocol
1) Standard Cycling Protocol
| Cov tshuaj tiv thaiv theem | Kub | Sijhawm | Lub voj voog | |
| 1 | Rov qab transcription | 50 ° Ca | 10 min | 1 |
| 2 | Thawj denaturation | 95 ° C | 5 feeb | 1 |
| 3 | Amplification cov tshuaj tiv thaiv | 95 ° C | 15 sec | 45 cycles |
| 60 ° Cb | 30 secc |
2) Ceev Cycling raws tu qauv
|
| Cov tshuaj tiv thaiv theem | Kub | Sijhawm | Lub voj voog |
| 1 | Rov qab transcription | 50 ° Ca | 2 min | 1 |
| 2 | Thawj denaturation | 95 ° C | 2 sec | 1 |
| 3 | Amplification cov tshuaj tiv thaiv | 95 ° C | 1 sec |
45 cycles |
| 60 ° Cb | 13 secc |
Nco tseg:
a) Rov qab kev sau ntawv: Qhov kub tuaj yeem xaiv 42 ° C lossis 50 ° C rau 10-15 feeb.
b) Amplification cov tshuaj tiv thaiv: Qhov ntsuas kub tau hloov kho raws li tus nqi Tm ntawm tus tsim primers.
c)Fluorescence teeb liab nrhiav tau: Thov teeb tsa cov txheej txheem sim raws li qhov yuav tsum tau ua ntawm covinstrument manual.
Cov Ntaub Ntawv Qhia Txog Kev Ua Haujlwm / Specifications
| Pib kub | Built-in kub pib |
| Kev kuaj xyuas txoj kev | Primer-probe detection |
| PCR txoj kev | Ib kauj ruam RT-qPCR |
| Hom qauv | RNA |
Sau ntawv
1. Cov khoom no yog siv rau kev tshawb fawb nkaus xwb.
2. Thov hnav PPE tsim nyog, xws li lub tsho tiv no thiab hnab looj tes, kom paub tseeb tias koj noj qab haus huv thiab kev nyab xeeb!
