Ib Kauj Ruam RT-qPCR cov khoom siv sojntsuam
Kev piav qhia
Ib Kauj Ruam QRT-PCR Probe Cov Khoom Siv yog tsim tshwj xeeb rau qPCR uas ncaj qha siv RNA (xws li tus kab mob RNA) ua qauv.Siv cov gene tshwj xeeb primers (GSP), qhov kev hloov pauv rov qab thiab qPCR tuaj yeem ua tiav hauv ib lub raj, txo cov txheej txheem pipetting thiab kev pheej hmoo ntawm kev sib kis.Nws tuaj yeem ua haujlwm tsis tau ntawm 55 ℃ yam tsis muaj kev cuam tshuam rau kev ua haujlwm thiab rhiab heev ntawm qRT-PCR.Ib Kauj Ruam QRT-PCR Probe Kit yog muab rau hauv Master Mix.Lub 5 × Ib Kauj Ruam Sib xyaw muaj qhov ua kom zoo tsis zoo thiab dNTP / dUTP Mix, thiab tsim nyog rau kev kuaj xyuas qhov tshwj xeeb raws li cov tshuaj fluorescence sau npe probes (xws li TaqMan).
Basic Principles ntawm RT-qPCR
Kev qhia tshwj xeeb
| Cov khoom kuaj | Specification | Kev tshwm sim |
| (SDS PAGE) Purity of Enzyme Stock (SDS PAGE) | ≥95% | Hla |
| Endonuclease Activity | Tsis pom | Hla |
| Kev ua Exodulease | Tsis pom | Hla |
| Rnase Activity | Tsis pom | Hla |
| Residual E.coli DNA | 1copies/60 | Hla |
| Functional Assay-System | 90% ≤ 110% | Hla |
Cheebtsam
| Cheebtsam | 100 rxn | 1,000 rns | 5,000 rx |
| RNase-dawb ddH2O | 2 * 1ml | 20 ml | 100 ml |
| 5 * ib kauj ruam sib xyaw | 600l ua | 6 * 1ml | 30 ml |
| Ib kauj ruam enzyme mix | 150l ua | 2 * 750 hli | 7.5ml ib |
| 50 * ROX siv Dye 1 | 60l ua | 600l ua | 3 * 1ml |
| 50 * ROX siv Dye 2 | 60l ua | 600l ua | 3 * 1ml |
a.Ib-kauj ruam tsis muaj xws li dNTP Mix thiab Mg2+.
b.Enzyme Mix feem ntau muaj rov qab
transcriptase, Kub Start Taq DNA polymerase (antibody modification) thiab RNase inhibitor.
c.Siv los kho qhov yuam kev ntawm fluorescene sinals ntawm cov qhov dej sib txawv.
d.ROX: Koj yuav tsum xaiv qhov ntsuas ntsuas raws li tus qauv ntawm cov ntsuas ntsuas.
Daim ntawv thov
QPCR nrhiav pom
Shipping thiab Cia
Shipping:Cov hnab ntim dej khov
Kev cia khoom:Khaws ntawm -20 ℃.
Shief Lub Neej:18 hli
