Cov roj (cholesterol) oxidase (COD/CHOD)
Kev piav qhia
Cholesterol oxidase (CHOD) catalyzes thawj kauj ruam hauv cov roj cholesterol catabolism.Qee cov kab mob uas tsis yog kab mob, xws li Streptomyces tuaj yeem siv cov roj cholesterol ua cov pa roj carbon.Cov kab mob pathogenic, xws li Rhodococcus equi, yuav tsum tau CHOD kom kis tus tswv lub macrophage.CHOD yog bifunctional.Cholesterol yog pib oxidized rau cholest-5-en-3-ib qho hauv FAD-yuav tsum tau kauj ruam.Lub cholest-5-en-3-ib yog isomerized rau cholest-4-en3-one.Cov tshuaj tiv thaiv isomerization tej zaum yuav rov qab ib nrab.Qhov kev ua ntawm CHOD nyob ntawm seb lub cev muaj zog ntawm daim nyias nyias uas lub substrate yog khi.
CHOD yog siv los txiav txim siab cov roj cholesterol.Nws yog qhov thib ob feem ntau siv enzyme hauv kev kuaj mob tom qab qabzib oxidase.CHOD kuj pom daim ntawv thov hauv microanalysis ntawm cov tshuaj steroids hauv cov qauv zaub mov thiab qhov txawv ntawm 3-ketosteroids los ntawm 3b-hydroxysteroids.Transgenic nroj tsuag qhia txog cov roj cholesterol oxidase raug tshawb xyuas hauv kev tawm tsam cov paj rwb boll weevil.Cov roj (cholesterol) oxidase kuj tau siv los ua kev sojntsuam molecular los elucidate cellular membrane qauv.
Chemical Structure
Reaction Principple
Cov roj (cholesterol) + O2 →△4-Cholesten-3-ib + H2O2
Specification
| Cov khoom kuaj | Specifications |
| Kev piav qhia | Yellowish amorphous hmoov, lyophilized |
| Kev ua si | ≥8U/mg |
| Purity (SDS-PAGE) | ≥90% |
| Solubility (10mg hmoov / ml) | Ntshiab |
| Catalase | ≤ 0.001% |
| Glucose oxidase | ≤0.01% |
| Cov roj (cholesterol) esterase | ≤0.01% |
| ATPase | ≤ 0.005% |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Shipped qis dua -15 ° C
Cia:Khaws ntawm -25 ~ -15 ° C (Lub Sij Hawm Ntev), 2-8 ° C (Lub Sij Hawm Luv)
Pom zoo rov sim duaLub neej:1 xyoo
