Deoxyribonuclease I (Dnase I)
Kev piav qhia
DNase I (Deoxyribonuclease I) yog ib qho endodeoxyribonuclease uas tuaj yeem zom ib leeg- lossis ob-kab DNA.Nws paub thiab cleaves phosphodiester bonds los tsim monodeoxynucleotides los yog ib leeg-los yog ob-stranded oligodeoxynucleotides nrog phosphate pawg ntawm 5'-terminal thiab hydroxyl ntawm 3'-terminal.Qhov kev ua ntawm DNase kuv nyob ntawm Ca 2+ thiab tuaj yeem qhib tau los ntawm divalent hlau ions xws li Mn 2+ thiab Zn 2+ .5 mM Ca 2+ tiv thaiv cov enzyme los ntawm hydrolysis.Nyob rau hauv lub xub ntiag ntawm Mg 2+, lub enzyme yuav randomly paub thiab cleave txhua qhov chaw ntawm ib tug strand ntawm DNA.Nyob rau hauv lub xub ntiag ntawm Mn 2+ , ob txoj hlua ntawm DNA tuaj yeem raug lees paub ib txhij thiab cleaved ntawm yuav luag tib qhov chaw los tsim cov kab kawg DNA tawg lossis nplaum kawg DNA tawg nrog 1-2 nucleotides protruding.
Chemical Structure
Unit txhais
Ib chav tsev yog txhais raws li tus nqi ntawm cov enzyme uas yuav ua kom tiav 1 µg ntawm pBR322 DNA hauv 10 feeb ntawm 37 ° C.
Specification
| Cov khoom kuaj | Specifications |
| Purity (SDS-PAGE) | ≥ 95% |
| Rnase Activity | Tsis muaj Degradation |
| gDNA Cov kab mob | ≤ 1 daim / μL |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Shipped hauv qab 0 ° C
Cia:Khaws ntawm -25 ~ -15 ° C
Pom zoo rov xeem lub neej:2 xyoo
