Ultra Nuclease
Kev piav qhia
UltraNuclease yog ib qho kev tsim kho caj ces endonuclaese muab los ntawm Serratia marcescens, uas muaj peev xwm degrade DNA los yog RNA, ob leeg los yog ib leeg stranded, linear los yog ncig nyob rau hauv ntau yam mob, tag nrho degrade nucleic acids rau hauv 5'-monophosphate oligonucleotides nrog 3-5. puag ntev.
Tom qab kev hloov kho genetic engineering, cov khoom tau fermented, qhia, thiab purified nyob rau hauv Escherichia coli (E. coli), uas txo cov viscosity ntawm cell supernatant thiab cell lysate nyob rau hauv kev tshawb fawb, tab sis kuj txhim kho cov purification efficiency thiab functional tshawb fawb ntawm cov protein.Nws kuj tuaj yeem siv rau hauv kev kho noob, kho tus kab mob, kev tsim tshuaj tiv thaiv, protein thiab polysaccharide kev lag luam kws tshuaj ua tus tswv tsev residue nucleic acid tshem tawm reagent.
Chemical Structure
Unit txhais
Tus nqi ntawm cov enzyme siv los hloov cov nqi nqus ntawm △A260 los ntawm 1.0 hauv 30min ntawm 37 ° C, pH 8.0, sib npaug rau digested 37μg salmon phev DNA los ntawm kev txiav rau hauv oligonucleotides, tau txhais tias yog ib chav ua haujlwm.
Kev siv thiab siv
• Tshem tawm cov exogenous nucleic acid los ntawm cov khoom siv tshuaj tiv thaiv, txo qhov kev pheej hmoo ntawm residual nucleic acid toxicity thiab txhim kho cov khoom ruaj ntseg.
• Txo cov viscosity ntawm cov kua pub los ntawm nucleic acid, txo lub sij hawm ua thiab ua kom cov protein ntau.
• Tshem tawm cov nucleic acid uas qhwv cov kab mob (tus kab mob, suav nrog lub cev, thiab lwm yam), uas yog qhov tsim nyog rau kev tso tawm thiab ua kom huv ntawm cov khoom.
• Kev kho mob Nuclease tuaj yeem txhim kho qhov kev daws teeb meem thiab rov qab los ntawm cov qauv rau kab chromatography, electrophoresis thiab kev tsom xam blotting.
• Hauv kev kho noob caj noob ces, nucleic acid raug tshem tawm kom tau txais cov kab mob purified adeno-associated.
Specification
| Cov khoom kuaj | Specifications |
| Kev piav qhia | Ntshiab thiab tsis muaj xim |
| Kev ua si | ≥ 250 U / ul |
| Cov haujlwm tshwj xeeb | ≥1.1 * 106U/mg |
| Purity (SDS-PAGE) | ≥ 99.0% |
| Proteases | Tsis pom muaj leej twg |
| Bioburden | 10 cfu / 100,000U |
| Endotoxines (LAL-test) | 0.25EU / 1,000U |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Shipped hauv qab 0 ° C
Cia:Khaws ntawm -25 ~ -15 ° C
Pom zoo rov xeem lub neej:2 xyoos (tsis txhob khov-thawing)
