DNase I (Rnase Free) (2u/ul)
Cat No: HC4007B
DNase I yog ib qho endonuclease uas zom tau ib leeg-los yog ob-stranded DNA.Nws tuaj yeem hydrolyze phosphodiester bonds los tsim cov mono-thiab oligodeoxynucleotides uas muaj 5'-phosphate pawg thiab 3'-OH pawg.Qhov zoo tshaj plaws pH ua haujlwm ntawm DNase I yog 7-8.Qhov kev ua ntawm DNase kuv nyob ntawm Ca2+thiab tuaj yeem qhib tau los ntawm divalent hlau ions xws li CO2, Mn2+, Zn2+, thiab lwm yam Nyob rau hauv lub xub ntiag ntawm Mg2+, DNase Kuv tuaj yeem random cleave txhua qhov chaw ntawm ob-stranded DNA;Thaum Mn2+, DNase Kuv tuaj yeem cleave DNA ob-stranded ntawm tib qhov chaw, tsim blunt kawg lossis nplaum kawg nrog 1-2 nucleotides protruding.Nws tuaj yeem siv rau kev ua ntawm ntau yam RNA cov qauv.
Cheebtsam
| Lub npe | 1 KU | 5 KUB |
| Recombinant DNaseI (RNase-dawb) | 500 mL | 5 × 500 hli |
| DNase I Reaction Buffer (10 ×) | 1 ml ib | 5 × 1ml |
Cia tej yam kev mob
Cov khoom no yuav tsum khaws cia ntawm -25 ~ -15 ℃ rau 2 xyoos.Thov tsis txhob rov khov-thaw.
Cov lus qhia
Siv rau kev tshem tawm DNA ntawm RNA cov qauv rau kev siv nkaus xwb.
1. Thov siv RNase-free centrifuge raj thiab pipette cov lus qhia los npaj cov tshuaj tiv thaiv hauv qab no:
| Cheebtsam | Ntim (μL) |
| DNase I Reaction Buffer (10 ×) | 1 |
| Recombinant DNasel (RNase-dawb) | 1 |
| RNA | X |
| RNase-dawb ddH2O | Mus txog 10 |
2. Cov kev mob tshwm sim yog raws li nram no: 37 ℃, tom qab 15-30 mins, ntxiv qhov kawg concentration ntawm 2.5 mM EDTA tov thiab sib tov zoo, ces 65 ℃ rau 10 mins.Cov txheej txheem txheej txheem tuaj yeem siv rau kev sim tom qab RT-PCR lossis RT-qPCR, thiab lwm yam.
Sau ntawv
1. DNase l yog rhiab rau lub cev denaturation;Thaum sib tov, maj mam thim rov qab lub xeem raj thiabco zoo, tsis txhob co zog.
2. Cov enzyme yuav tsum tau muab cia rau hauv lub thawv dej khov lossis hauv lub da dej dej khov thaum siv, thiab yuav tsum khaws cia ntawm -20 ℃ tam sim tom qab siv.
3. Cov khoom no yog siv rau kev tshawb fawb nkaus xwb.
4. Thov ua haujlwm nrog cov tsho loj thiab cov hnab looj tes pov tseg, kom koj muaj kev nyab xeeb.
