Rnas A
Ribonuclease A (RNaseA) yog ib leeg-stranded polypeptide uas muaj 4 disulfide bonds nrog ib tug molecular hnyav li ntawm 13.7 kDa.RNase Ais yog ib qho endoribonuclease uas tshwj xeeb degrades ib leeg-stranded RNA ntawm C thiab U residues.Tshwj xeeb, qhov cleavage paub txog phosphodiester daim ntawv cog lus tsim los ntawm 5'-ribose ntawm nucleotide thiab phosphate pawg ntawm 3'-ribose ntawm pyrimidine nucleotide uas nyob ib sab, yog li ntawd 2,3'-Cyclic phosphates yog hydrolyzed rau 3 sib xws. 'nucleoside phosphates (piv txwv li, pG-pG-pC-pA-pG yog cleaved los ntawm RNase A los tsim pG-pG-pCp thiab A-PG).RNase A yog qhov tseem ceeb tshaj plaws hauv kev tshem tawm ib leeg-stranded RNA.Pom zoo ua hauj lwm concentration yog 1- 100 μ G / mL, sib xws nrog ntau yam tshuaj tiv thaiv kab mob.Tsawg ntsev concentration (0-100 mM NaCl) tuaj yeem siv los txiav ib leeg-stranded RNA, ob-stranded RNA, thiab RNA chains tsim los ntawm RNA-DNA hybridization.
Txawm li cas los xij, ntawm cov ntsev siab (≥0.3 M), RNase A tsuas yog tshwj xeeb cleaves ib leeg-stranded RNA.
RNase A feem ntau yog siv los tshem tawm RNA thaum lub sijhawm npaj ntawm plasmid DNA lossis genomic DNA.Txawm hais tias DNase tsis ua haujlwm thaum lub sijhawm npaj tuaj yeem cuam tshuam cov tshuaj tiv thaiv.Cov txheej txheem ntawm boiling nyob rau hauv ib da dej yuav siv tau los inactivate DNase kev ua ub no.Cov khoom no tsis muaj DNase thiab protease, thiab tsis xav tau kev kho cua sov ua ntej siv.Tsis tas li ntawd, cov khoom no kuj tuaj yeem siv rau hauv kev sim tshuaj lom neeg biology xws li RNase tiv thaiv kev tsom xam thiab RNA kev soj ntsuam ib ntus.
Cia tej yam kev mob
Cov khoom yuav tsum khaws cia ntawm -25 ℃ ~ - 15 ℃ rau 2 xyoos.
Specifications
| Qhov tshwm sim | Hmoov |
| Ntau | 100 mg / 1 g |
| Yam khoom | RNase A |
Cov lus qhia
Qhov no yog ib txoj hauv kev los npaj RNase A cia tshuaj.Nws kuj tuaj yeem npaj taulos ntawm lwm txoj hauv kev raws li cov txheej txheem ib txwm siv hauv chav kuaj lossis cov ntaub ntawv siv (xws lincaj qha dissolving hauv 10 mM Tris-HCl, pH 7.5 los yog Tris-NaCl tov)
1. Siv 10 mM sodium acetate (pH 5.2) los npaj 10 mg/mL ntawm RNase A cia tshuaj.
2. Cua sov ntawm 100 ℃ rau 15 min.
3. Txias rau hauv chav sov, ntxiv 1/10 ntim ntawm 1 M Tris-HCl (pH 7.4), kho nws cov pH rau 7.4 (rauPiv txwv li, ntxiv 500 mL ntawm 10 mg / mL RNase cia tshuaj 1 M Tris-HCl, pH7.4).
4. Sub-packaged ntawm -20 ℃ rau khov cia, uas tuaj yeem ruaj khov mus txog 2 xyoos.
[Notes]:
Thaum boiling RNaseA tov nyob rau hauv nruab nrab tej yam kev mob, RNase nag yuav tsim;Boil nws ntawm qis pH, thiab yog tias muaj nag lossis daus, nws tuaj yeem pom, uas tuaj yeem tshwm sim los ntawm qhov muaj protein ntau impurities.Yog tias cov av pom tom qab boiling, impurities tuaj yeem raug tshem tawm los ntawm kev kub ceev centrifugation (13000rpm), thiab tom qab ntawd sub-packed rau khov cia.
Sau ntawv
Thov hnav PPE tsim nyog, xws li lub tsho tiv no thiab hnab looj tes, kom ntseeg tau tias koj noj qab haus huv thiab kev nyab xeeb.
