Ribonuclease III (RNase III)
Kev piav qhia
Cov khoom no yog ribonuclease III (RNase III) recombinantly qhia los ntawm E. coli.Qhov tshwj xeeb exonuclease cleaves ob-stranded RNA (dsRNA) thiab tsim 12-35bp dsRNA fragments nrog 5'-PO4 thiab 3'-OH, 3' overhangs
Chemical Structure
Unit txhais
Lub luag haujlwm chav tsev txhais: Ib chav yog hais txog tus nqi ntawm cov enzyme uas yuav tsum tau degrade 1 μg ntawm
dsRNA rau siRNA hauv 50 μL cov tshuaj tiv thaiv ntawm 37 ° C rau 20 feeb.
Specification
Cov khoom kuaj | Specifications |
Kev Ua Haujlwm Exonuclease | Tso tawm < 0.1% ntawm tag nrho cov xov tooj cua |
Tsis-Specific Dnase Activity | Tsis ntes tau |
Protein Purity Assay (SDS-PAGE) | ≥ 95% |
RNase Kev Ua Haujlwm (Ntev Digestion) | > 90% ntawm cov substrate RNA tseem nyob twj ywm |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Dej khov qhuav
Cia:Khaws ntawm -25 ~ -15 ° C
Pom zoo rov xeem lub neej:2 xyoo
cov khoom muaj feem xyuam
Sau koj cov lus ntawm no thiab xa tuaj rau peb