ELEISA KIT rau Trypsin
Kev piav qhia
Recombinant Trypsin nquag siv hauv biopharmaceutical manufacturing-thaum lub sijhawm npaj ntawm tes lossis rau kev hloov kho thiab ua kom cov khoom.Trypsin ua rau muaj kev pheej hmoo ntawm kev nyab xeeb thiab yog li yuav tsum tau muab tshem tawm ua ntej qhov khoom kawg tso tawm.Cov Khoom Siv Sandwich no yog rau kev ntsuas ntau ntawm cov seem Trypsin hauv cell culture supernatant thiab lwm cov txheej txheem hauv biopharmaceutical manufacturing thaum Trypsin siv.
Cov khoom siv no yog Enzyme-Linked Immunosorbent Assay (ELISA).Lub phaj tau ua ntej coated nrog Porcine trypsin antibody.Trypsin tam sim no nyob rau hauv cov qauv yog ntxiv thiab khi rau cov tshuaj tiv thaiv coated rau ntawm qhov dej.Thiab tom qab ntawd biotinylated Porcine trypsin antibody ntxiv thiab khi rau trypsin hauv cov qauv.Tom qab ntxuav, HRP-Streptavidin ntxiv thiab khi rau Biotinylated trypsin antibody.Tom qab incubation unbound HRP-Streptavidin yog ntxuav tawm.Tom qab ntawd TMB substrate tov yog ntxiv thiab catalyzed los ntawm HRP los tsim cov khoom xim xiav uas hloov mus rau daj tom qab ntxiv acidic nres.Qhov ceev ntawm daj yog proportional rau lub hom phiaj tus nqi ntawm trypsin
qauv ntes nyob rau hauv phaj.Lub absorbance yog ntsuas ntawm 450 nm.
Chemical Structure
Specification
Cov khoom kuaj | Specifications |
Qhov tshwm sim | Ua kom tiav packing thiab tsis muaj kua xau |
Kev txwv qis ntawm kev kuaj pom | 0.003 ng/ml |
Tsawg qhov txwv ntawm qhov ntau | 0.039 ng/ml |
Precision | Intra assay CV ≤10% |
Kev thauj mus los thiab khaws cia
Kev thauj mus los:Ambient
Cia:Yuav khaws cia ntawm -25 ~ -15 ° C hauv txee lub neej, 2-8 ° C rau lwm yam kev sim yooj yim
Pom zoo rov xeem lub neej:1 xyoo