Superstart Taq DNA Polymerase

Superstart Taq DNA Polymerase yog qhov kub pib DNA polymerase.Cov khoom no tuaj yeem tsis tsuas yog zoo dua inhibit qhov tsis muaj cov tshuaj tiv thaiv tshwj xeeb los ntawm qhov tsis yog tshwj xeeb annealing ntawm primers los yog primer aggregation nyob rau hauv tus txheej txheem ntawm PCR system npaj thiab amplification.Yog li ntawd, nws tuaj yeem ua tiav cov txiaj ntsig zoo hauv ntau qhov kev nthuav dav.Tsis tas li ntawd, nws tuaj yeem ua kom zoo dua qhov kev nthuav dav ntawm cov qauv uas tsis tshua muaj siab kom tau txais cov khoom lag luam ntau dua thiab ua tiav cov txiaj ntsig ruaj khov thiab huab cua loj.

Cheebtsam

1.5 U/μL Superstart Taq DNA polymerase

2.10 × PCR Buffer II (Mg² + dawb) (yeem)

3.25 mMgcl₂(yeem)

* 10 × PCR Buffer II (Mg²+ pub dawb) tsis muaj dNTP thiab Mg²+, thov ntxiv dNTPs thiab MgCl₂thaum npaj cov tshuaj tiv thaiv kab mob.

Cov ntawv thov pom zoo

1.Kev tshawb nrhiav African swine fever.

2.Kev kuaj mob STI.

3.Multiplex amplification.

Kev cia khoom

-20 ° C rau lub sij hawm ntev cia, yuav tsum tau tov zoo ua ntej siv, tsis txhob nquag khov-thaw.

* Yog tias nag lossis daus tshwm sim tom qab lub tub yees, nws yog qhov qub;nws raug pom zoo kom sib npaug rau chav tsev kub ua ntej sib tov thiab siv.

Unit txhais

Ib chav ua haujlwm (U) txhais tau tias yog tus nqi ntawm cov enzyme uas suav nrog 10 nmol ntawm deoxyribonucleotide rau hauv cov khoom siv acid-insoluble ntawm 74 ° C rau 30mins siv cov activated salmon phev DNA ua qauv / primer.

Kev Tswj Xyuas Zoo

1.SDS-PAGE electrophoretic purity ntau dua 98%.

2.Amplification rhiab heev, batch-to-batch tswj, stability.

3.Tsis muaj exogenous nuclease kev ua, tsis muaj exogenous endonuclease los yog exonuclease paug

Cov lus qhia

Kev teeb tsa kev daws teeb meem

Nco tseg:

1) a.Qhov tsis muaj dNTP thiab Mg² +, thov ntxiv dNTPs thiab MgCl₂thaum npaj cov tshuaj tiv thaiv kab mob.

2) b.Yog siv rau qPCR/qRT-PCR, fluorescent probes yuav tsum tau ntxiv rau cov tshuaj tiv thaiv.Feem ntau, qhov kawg primer concentration ntawm 0.2 μM yuav muab tau zoo;Yog tias cov tshuaj tiv thaiv kev ua haujlwm tsis zoo, cov primer concentration tuaj yeem hloov kho hauv thaj tsam ntawm 0.2-1 μM.Kev sojntsuam concentration yog

3) feem ntau optimized nyob rau hauv thaj tsam ntawm 0.1-0.3 μM.Concentration gradient thwmsim tuaj yeem ua tau los nrhiav qhov zoo tshaj plaws ua ke ntawm primer thiab sojntsuam.

Thermal cycling raws tu qauv

Sau ntawv

1.Nws tuaj yeem txais 95 ℃ lossis 94 ℃, 1 ~ 5 mins pib kub ceev.

2.Lub system yog adaptable heev, nrog ntau dua specification thiab rhiab heev.

3.Hauv PCR ib txwm, nws tuaj yeem tau txais cov khoom lag luam ntau dua, thiab hauv fluorescence quantitative PCR, nws tuaj yeem txhim kho qhov normalization ntawm amplification nkhaus thiab tus nqi fluorescence ntawm tus qauv nrog tsawg concentration.Haum rau siv raws li lub siab rhiab heev PCR nrhiav reagent.

4.thiab tuaj yeem siv rau hauv multiplex PCR amplification tshwm sim.

5.5′ → 3′ kev ua haujlwm polymerase, 5′ → 3′ kev ua haujlwm exonuclease;tsis muaj 3′→ 5′ exonuclease kev ua;tsis muaj proofreading function.

6.Tsim nyog rau kev kuaj kom zoo thiab ntau ntawm PCR thiab RT-PCR.

7.Qhov 3' kawg ntawm cov khoom PCR yog A, uas tuaj yeem ncaj qha cloned rau hauv T vector.

8.Txoj kev peb-kauj ruam yog pom zoo rau primers uas tsis tshua muaj annealing kub los yog rau amplification ntawm fragments ntev tshaj 200 bp.